LifeTein - Anti-HEL (Hen Egg Lysozyme) Human IgG1 isotype control

Origin:

CHO-S cell

Application:

The anti HEL (Hen Egg Lysozyme) IgG isotype control antibodies were expressed in CHO-S cells and purified with protein A affinity column. The light chain is kappa with the whole mouse variable domain (107 amino acids) while the heavy chain contains the whole mouse variable domain (116 A.A). The isotype control antibodies can be used as negative controls to differentiate non-specific background signal from your target antibody signals. Whole molecule immunoglobulins may also be used as standard protein or coating protein in ELISA, Western-Blot, immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and flow cytometry (FACS). This product is also suitable for various in vitro and in vivo studies which require low endotoxin level. LifeTein`s isotype control proteins were produced under stringent endotoxin control conditions of <0.5 EU/μg.

Quality Control:

Endotoxin level: <0.5 EU/mg by the LAL method

Purity: >95% by HPLC and SDS-PAGE

Purification Method:

Protein A affinity column

Concentration:

1 mg, Lyophilized in 20 mM PBS, pH7.4

Conjugation:

Unconjugated

Storage:

Store at -80°C in PBS for up to 2 years. Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 1mg/ml. Aliquot to avoid repeated freeze-thaw cycles.