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Handy FluorCam - Model FC 1000-H -Lightweight Portable Chlorophyll Fluorescence Imaging Device
Handy FluorCam FC 1000-H is a lightweight, portable device designed for time resolved chlorophyll ?uorescence imaging of leaves, small plants, leaf segments, mosses, lichens, seeds, roots, tissues on plates or algal colonies both in a field and laboratory. The typical size of an investigated object is up to 41.5 × 31.5 mm. Handy FluorCam is a compact instrument consisting of a CCD camera and four fixed LED panels, one pair supplying the measuring pulses and the second pair providing actinic illumination and saturating ?ash. Handling of larger leaves (e.g., tobacco) is simplified if an additional leaf clip with gentle lock/release mechanism is mounted. The Handy FluorCam FC 1000-H is an easily portable system, it can be powered from the power supply or it can run on batteries provided in a convenient bag carried on the shoulder.
Handy FluorCam FC 1000-H generates images of fluorescence signal at any moment of the experiment and presents them using
a false color scale. Full kinetic analysis is available. In all applications, the camera allows imaging of fluorescence transients that are induced by actinic light or by saturating flashes. The timing and amplitude of actinic irradiance are determined by user-defined protocols. The Handy FluorCam is delivered with a laptop computer preinstalled with a comprehensive software package comprising full system control, Wizard with the most frequently used experimental protocols, data acquisition and image processing. For an experienced professional, the software offers a sophisticated programming language that can be used for designing novel timing and measuring sequences.
- Four super bright LED light panels 40 x 40 mm
- Imaged area 35 x 46 mm
- Excitation light colors:
- measuring pulses: red-orange (620 nm)
- actinic / saturating light: white – standard version, blue (455 nm), red-orange (620 nm)
- far-red 735 nm
- white: up to 3,900 µmol.m-2. s-1
- blue: up to 4,900 µmol.m-2. s-1
- red-orange: up to 3,800 µmol.m-2. s-1
- white: approx. 1,000 µmol.m-2. s-1
- blue: approx. 1,400 µmol.m-2. s-1
- red-orange: approx. 800 µmol.m-2. s-1
- Light regime:
- Static or sinus form (hub connected)
- FV/FM
- Kautsky induction
- Quenching analysis
- Light Curve
- Measured parameters: FO, FM, FV, FO`, FM`, FV`, FT
- More than 50 calculated parameters: FV/FM, FV`/FM`, PhiPSII , NPQ, qN, qP, Rfd, ETR (via Light Curve protocol), PAR-absorptivity coefficient, and many others
- Leaves, small plants, fruits, vegetables
- Mosses, lichens
- Cyanobacteria, green algae
- Nematodes
- Predefined imaging masks for 384-well plate, 96-well plate, Petri dish, etc.
- Screening for photosynthetic performance and metabolite perturbations
- Detection of biotic and abiotic stress
- Plant’s resistance or susceptibility to various stress factors
- Plant-microbe interactions
- Agriculture and horticulture
- Yield improvement
- Fully automated control of the whole FluorCam system
- Image acquisition via automated experimental protocols:
- numerous predefined protocols
- possibility to create user defined protocols via a program Wizard
- multiple (automatically repeated) experiments
- barcode reader support
- automatic or manual image segmentation – e.g., labeling of individual plants
- analysis of kinetic data from all samples within the field of view
- numerous image manipulation tools
- export to text file, avi, bmp or raw data formats
- Windows 7, or higher compatible
Fluorescence Parameters:
- Measured parameters: F0, FM, FV, F0`, FM`, FV`, FT
- More than 50 calculated parameters: FV/FM, FV`/FM`, PhiPSII, NPQ, qN, qP, Rfd, PAR-absorptivity coefficient, and many others
Excitation Light Sources:
Measuring pulses:red-orange (620 nm);
Actinic/saturating light:white (standard),red-orange (620 nm) or blue (455 nm)
Saturating Pulse Illumination:
White: Up to 3,900 µmol.m-2. s-1
Blue: Up to 4,900 µmol.m-2. s-1
Red-orange: Up to 3,800 µmol.m-2. s-1
Actinic Illumination:
White: 1,000 µmol.m-2. s-1
Blue: Up to 1,400 µmol.m-2. s-1
Red-orange: Up to 800 µmol.m-2. s-1
Light Regime:
Static or sine waveform (hub connected)
Custom-Defined Protocols:
Variable timing, special language and scripts
Handy FluorCam Weight:
1.8 kg
Leaf clip Weight:
0.2 kg
Power Supply Weight:
2.5 kg
FluorCam Stand Weight:
1.5 kg
Notebook Weight (incl. all accessories):
3.5 kg
Power Input:
Max. 200 W
Electrical:
90 – 260 V
Dimensions
(W × D × H): 151 × 151 × 263 mm, 280 mm height with leaf clip
Cameras:
High-sensitivity TOMI-1
or high-resolution TOMI-2