Abcam - Luminescent ATP Detection Assay Kit
Luminescent ATP Detection Assay Kit (ab113849) is used to measure the level of ATP within the cell. The luminescent ATP assay protocol involves lysis of the cell sample, addition of luciferase enzyme and luciferin, and measurement of the emitted light using a tube or microplate-based luminometer.
- Detection method
Luminescent
- Sample type
Adherent cells, Suspension cells
- Assay type
Quantitative
- Assay time
0h 30m
This kit irreversibly inactivates ATP degrading enzymes (ATPases) during the lysis step, ensuring that the luminescent signal obtained truly corresponds to the endogenous levels of ATP.
Luminescent ATP assay protocol summary:add ATP standard into standard wells and media into control wells in same plate containing cells to be analyzed
add detergent solution and incubate for 5 min to lyse cells and stabilize ATP
add substrate solution and incubate for 5 min
store plate in dark for 10 min
analyze on luminescence plate reader
Notes
Total levels of cellular ATP can be used to assess cell viability, cell proliferation and cytotoxicity of a wide range of compounds and biological response modifiers.
We also offer a very popular alternative colorimetric/fluorometric ATP assay kit ab83355 based on the phosphorylation of glycerol.
Related assays
Review the cell health assay guide to learn about kits to perform a cell viability assay, cytotoxicity assay and cell proliferation assay.
Review the metabolism assay guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
