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MedChemExpressModel 4-Methylumbelliferyl-ß-D-glucuronide -6160-80-1

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4-Methylumbelliferyl-β-D-glucuronide is a fluorogenic substrat (λex=362 nm, λem=445 nm). 4-Methylumbelliferyl-β-D-glucuronide has potential applications in detecting the activity of β-glucuronidase and the number of Escherichia coli[1][2][3].
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4-Methylumbelliferyl-β-D-glucuronide

MCE China:4-Methylumbelliferyl-β-D-glucuronide

Brand:MedChemExpress (MCE)

Cat. No.HY-D0935

CAS:6160-80-1

Storage:Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping:Room temperature in continental US; may vary elsewhere.

Description:4-Methylumbelliferyl-β-D-glucuronide is a fluorogenic substrat (λex=362 nm, λem=445 nm). 4-Methylumbelliferyl-β-D-glucuronide has potential applications in detecting the activity of β-glucuronidase and the number of Escherichia coli.

In Vitro:4-Methylumbelliferyl-β-D-glucuronide (156 µM-5 mM; 30 min) releases 4-methylumbelliferone at 37°C for enzymatic reactions[2]. 4-Methylumbelliferyl-β-D-glucuronide (50 µg/mL; 24 h) is used to detect the number of Escherichia coli in TSA medium[3].

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References:

[1]. George I, et al. Use of beta-D-galactosidase and beta-D-glucuronidase activities for quantitative detection of totaland fecal coliforms in wastewater. Can J Microbiol. 2001 Jul;47(7):670-5.  [Content Brief]

[2]. Sperker B, et al. High-performance liquid chromatographic quantification of 4-methylumbelliferyl-beta-D-glucuronide as a probe for human beta-glucuronidase activity in tissue homogenates. J Chromatogr B Biomed Appl. 1996 Oct 11;685(1):181-4.  [Content Brief]

[3]. Villari P, et al. An evaluation of the use of 4-methylumbelliferyl-beta-D-glucuronide (MUG) in different solid media for the detection and enumeration of Escherichia coli in foods. Lett Appl Microbiol. 1997 Apr;24(4):286-90.  [Content Brief]

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