MedChemExpress - Model 4-Methylumbelliferyl-ß-D-glucuronide hydrate -881005-91-0
4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat (λex=362 nm, λem=445 nm). 4-Methylumbelliferyl-β-D-glucuronide hydrate has potential applications in detecting the activity of β-glucuronidase and the number of Escherichia coli[1][2][3].MCE products for research use only. We do not sell to patients.
4-Methylumbelliferyl-β-D-glucuronide hydrate
MCE China：4-Methylumbelliferyl-β-D-glucuronide hydrate
Brand：MedChemExpress (MCE)
Cat. No.HY-D0935A
CAS：881005-91-0
Synonyms：MUG
Purity：99.92%
Storage：4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Shipping：Room temperature in continental US; may vary elsewhere.
Description：4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat (λex=362 nm, λem=445 nm). 4-Methylumbelliferyl-β-D-glucuronide hydrate has potential applications in detecting the activity of β-glucuronidase and the number of Escherichia coli.
In Vitro：4-Methylumbelliferyl-β-D-glucuronide hydrate (156 μM-5 mM; 30 min) releases 4-methylumbelliferone at 37℃ for enzymatic reactions[2].4-Methylumbelliferyl-β-D-glucuronide hydrate (50 μg/mL; 24 h) is used to detect the number of Escherichia coli in TSA medium[3].
Kinase Assay：GLUase activity of E. coli cells is measured. Three millilitres of 4-Methylumbelliferyl-β-D-glucuronide hydrate (MUGlu) solution (55 mg of hydrated MUGlu and 20 mL of Triton X-100 in 50 mL of sterile water) is added to each flask (final concentration:165 mg/L). The incubation temperature is 44°C. One hundred microlitres of 2 M NaOH solution is added to each 2.9-mL aliquot to obtain a pH>10 before the fluorescence measurement[1].
Emission (Em)：445
Excitation (Ex)：362
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References：
[1]. George I, et al. Use of beta-D-galactosidase and beta-D-glucuronidase activities for quantitative detection of totaland fecal coliforms in wastewater. Can J Microbiol. 2001 Jul;47(7):670-5. [Content Brief]
[2]. Sperker B, et al. High-performance liquid chromatographic quantification of 4-methylumbelliferyl-beta-D-glucuronide as a probe for human beta-glucuronidase activity in tissue homogenates. J Chromatogr B Biomed Appl. 1996 Oct 11;685(1):181-4. [Content Brief]
[3]. Villari P, et al. An evaluation of the use of 4-methylumbelliferyl-beta-D-glucuronide (MUG) in different solid media for the detection and enumeration of Escherichia coli in foods. Lett Appl Microbiol. 1997 Apr;24(4):286-90. [Content Brief]
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