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Thermolysin, Bacillus thermoproteolyticus rokko

MCE China：Thermolysin, Bacillus thermoproteolyticus rokko

Brand：MedChemExpress (MCE)

Cat. No.HY-P1748

CAS：9073-78-3

Synonyms：EC 3.4.24.27; TML

Storage：Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping：Room temperature in continental US; may vary elsewhere.

Description：Thermolysin, Bacillus thermoproteolyticus rokko (EC 3.4.24.27) (TML) is a thermostable neutral metalloproteinase enzyme secreted by the Gram-positive bacteria Bacillus thermoproteolyticus. Thermolysin catalyzes the hydrolysis of peptide bonds containing hydrophobic residues.Optimal pH: 8.0. Considerably stable from pH 5 to 9.5.Optimal temperature : 70 °C

In Vitro：Thermolysin (100 μg in 1 mL, 64°C for 4 h, in isolated starch granules) digestion results in the selective removal of a group of low-molecular-mass proteins ranging between 10 and 27 kD[2].Thermolysin can be used for the hydrolysis of bovine liver sarcoplasmic proteins[3]. Product InformationMolecular weight: 34.6 kDa (calculated) Optimum pH: 8 0, stable in the pH range of 5.0-9 5 Optimum temperature: 70℃ Isoelectric point: 4.45 Instructions Soluble in acetate buffer, pH 7.5 (0.2 mg/mL), forming a clear to slightly turbid colorless solution. Alternatively, use pure water or buffer according to the specific experimental reference.

In Vivo：Thermolysin (50 mg/kg, p.o., once) lowers systolic blood pressure in SHR rats[4].Thermolysin is not acutely toxic with an oral LD50 of more than 18000 mg/kg in rats and more than 24000 mg/kg in mice (Acute toxicity studies)[5].

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References：

[1]. Bertusvan den Burg, et al. Chapter 111 - Thermolysin and Related Bacillus Metallopeptidases.

[2]. Mu-Forster C, et al. Surface localization of zein storage proteins in starch granules from maize endosperm. Proteolytic removal by thermolysin and in vitro cross-linking of granule-associated polypeptides. Plant Physiol. 1998 Apr;116(4):1563-71.  [Content Brief]

[3]. Di Bernardini R, et al. Isolation, purification and characterization of antioxidant peptidic fractions from a bovine liver sarcoplasmic protein thermolysin hydrolyzate. Peptides. 2011 Feb;32(2):388-400.  [Content Brief]

[4]. Fujita H, et al: a prodrug-type ACE-inhibitory peptide derived from fish protein. Immunopharmacology. 1999 Oct 15;44(1-2):123-7.  [Content Brief]

[5]. Ke Q, et al. Safety evaluation of a thermolysin enzyme produced from Geobacillus stearothermophilus. Food Chem Toxicol. 2013 Sep;59:541-8.  [Content Brief]