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Meizheng - Model HE10101 -Bisphenol A (BPA) ELISA Test Kit
Bisphenol A (BPA) is a carbon-based synthetic compound with the chemical formula (CH3)2C(C6H4OH)2 belonging to the group of diphenylmethane derivatives and bisphenols, with two hydroxyphenyl groups. BPAis employed to make certain plastics and epoxy resins. BPA-based plastic is clear and tough, and is made into a variety of common consumer goods, such as water bottles, sports equipment, CDs, and DVDs. BPA can cause cancer and Neurological diseases.
- Product Name: Bisphenol A (BPA) ELISA Test Kit
- Catalog No.: HE10101
- Specification: 96T
- Storage Condition: 2-8℃
- Restriction: For research use only!
Use Principles
Bisphenol A ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The tetracycline antigen is precoated on the wells. The precoated antigen compete the tetracycline antibody (antibody solution) with tetracycline in the sample, anti- tetracycline antibody binds to the tetracycline-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the tetracycline concentration of the samples is derived.
Scope of Application
Qualitative or quantitative analysis the residues of Bisphenol A in plastic products, plastic packing bottles, water sample, milk powder, etc.
- Sensitivity, accuracy and precision
- Sensitivity: 2ppb
Limit of detection
- Plastic product (thickness＜0.5mm) 40ppb
- Plastic product (thickness＞0.5mm) 4ppb
- Water 4ppb
- Milk powder . 80ppb
- Percent Recovery, %
- Plastic product . 100±20%
- Water 85±10%
- Milk powder . 85±10%
- Precision (CV %): Intra-lab assay: CV%≤10%
Provided Materials and Reagent
- Component 96 wells
- Antigen coated plate 12 test strips of 8 wells each
- 6 vials of standard 1ml each
- High concentration standard 100ppm 1ml
- HRP-conjugate 12ml
- Antibody Solution 6ml
- Substrate A 6ml
- Substrate B 6ml
- Stop Solution 6ml
- Sample redissolving solution (8×) 40ml
- Concentrated Wash Buffer (10×) 40ml
- IFU 1
- Testing Report 1