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Microbial - Diagnostic Tools
Quantitative measurements of qPCR on the basis of DNA/RNA. qPCR is a quantitative measurement for both DNA and RNA and determines a targeted process or organism. In short, you already know what you are looking for and measure the quantity. For example, with qPCR you can determine how many fungi are present in your sample.
Traditional microbiological techniques are based on culturing microorganism. With this traditional technique you only select living and culturable organisms. Unfortunately, the majority of microorganisms (approximately 99%) are considered non-culturable. qPCR allows you look at the genetic material that is always present in a cell. That is why this technique gives a better reflection of the microorganisms that are actually present in the sample, compared to culturing.
An additional benefit to qPCR is that the sample can be fixated on the spot, so that the microbiology cannot change during transport.