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Distek BIOne Single-Use FermentorMicrobial Fermentation Applications

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The BIOne Single-Use Fermentor (SUF) by Distek is ideal for numerous microbial fermentation applications ranging from plasmid DNA production in E.coli to recombinant protein expression in yeast, meeting the extreme oxygen transfer demands of highly aerobic microbial cultures. Available in a 2L and 10L volume, the BIOne Single-Use Fermentor offers reduced turnaround time and seamless transitions between processes. Featuring an agnostic design compatible with most common bioprocessing controllers, the BIOne Single-Use Fermentor is ready-to-use out of the box.
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READY-TO-USE
 
The BIOne SUF, assembled in Distek’s ISO 7 cleanroom with USP Class VI materials and gamma sterilized, minimizes contamination risk. This closed system removes the need for cleaning, assembly, or autoclave sterilization and is ready-to-use out of the box.
 
FLEXIBLE
 
The BIOne SUF is ideal for a wide range of microbial applications, including plasmid production with E. coli, protein expression in yeast, and cultivated food development. The SUF is designed to operate under the same demanding conditions as comparable autoclavable vessels.
 
AGNOSTIC DESIGN
 
With a range of motor adapters available, the BIOne Single-Use Fermentor is compatible with many common bioprocessing controllers on the market. This allows for seamless integration into any microbial bioprocessing laboratory.
 
DESIGNED TO MEET YOUR DIVERSE MICROBIAL NEEDS WITH EASE
 
Discover our single-use fermentor solution for microbial applications, specifically designed to handle a variety of needs. Key features include dual Rushton impellers and a baffle cage for effective mixing, along with the option for an integrated single-use pH probe. Enjoy the benefits of a non-invasive DO (polarographic) port and a standard septum port for easy integration. Our weldable TPE tubing comes in various sizes to suit your process requirements. High capacity sparge and exhaust filters along with a flute sparger with 7 x 1.5 mm holes delivers excellent gas exchange for highly aerobic microbial cultures.
 
E. COLI GROWTH PROFILE: BIOne SUF vs. CONTROL
 
E. coli growth profiles were evaluated in a 2L BIOne Single-Use Fermentor (SUF) and glass vessel control for a 22-hour batch process. Both systems were operated with identical conditions. Similar growth and titer were observed in the BIOne SUF and glass vessel control. Performance results indicate that the BIOne SUF is a suitable bench scale model for microbial fermentation process development and material generation.