Microcystins, Coated Tube ELISA, 40-test
The Microcystins Tube Kit is an immunoassay for the quantitative and sensitive screening of Microcystins and Nodularins in water samples. This test is suitable for the quantitative and/or qualitative screening of Microcystins and Nodularins in drinking and recreational water samples. Samples requiring regulatory action should be confirmed by HPLC, GC/MS, or other conventional methods. The test is a direct competitive ELISA based on the recognition of Microcystins, Nodularins, and their congeners by specific antibodies.
The Microcystins, when present in a sample, and a Microcystins-enzyme conjugate compete for the binding sites of rabbit anti-Microcystins antibodies in solution. The Microcystins antibodies are then bound by a second antibody (anti-rabbit) immobilized on the test tube. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Microcystins present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.
- PN 520012A
- Format: Coated Tube Test