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Norgen Biotek - Model Cat. 21550 -Milk Bacterial DNA Isolation Kit
This kit provides a rapid spin column method for the isolation and purification of genomic DNA from both Gram-negative and Gram-positive bacteria in milk samples. It can also be used to process challenging milk samples such as Mastitic milk for example. The kit is highly sensitive and can isolate DNA from a cell density of as little as 10 bacteria contained in 1 mL of milk. Genomic DNA is isolated in 45 minutes, free of inhibitors and ready for any number of downstream applications including PCR, qPCR and Southern Blot analysis, sequencing and more.
For the rapid purification of genomic DNA from various bacterial species found in milk
- Genomic DNA can be isolated from as few as 10 bacterial cells in 1 mL of milk
- Isolate genomic DNA from both Gram-negative and Gram-positive bacteria in milk
- Can process challenging samples such as mastitic milk
- Inhibitor-free DNA is ready for PCR, qPCR, Southern Blot, sequencing & more
- Fast and efficient spin-column format
Kit Specifications
- Maximum Milk Input 1 mL
- Minimum Detection Limit 10 bacteria in 1 mL of milk
- Bacteria Species Processed Gram positive and Gram negative
- Time to Complete 10 Purifications 1 hour
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The Lysozyme should be stored at -20°C upon arrival, and the Resuspension Solution A should be stored at -20°C after addition of the lysozyme. The lyophilized Proteinase K should be stored at -20°C upon arrival and after reconstitution. These reagents should remain stable for at least 1 year in their unopened containers.