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- Alator - Model M110 - MMLV-Reverse ...
Alator - Model M110 -MMLV-Reverse Transcriptase
Source: E. coli. Size: Bulk. Concentration: 200 U/µl. Storage Buffer: 50 mM Tris-HCl (pH 7.6), 1 mM DTT, 0.1% NP-40, 0.1 mM EDTA, 150 mM NaCl and 50% glycerol.
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- Reaction Buffer (10X): 500mM Tris-HCl(pH8.3), 750mM KC1, 30mM MgCb and lOOmM DTT
- Unit Definition: One unit of the enzyme incorporates 1 nmol dTTP into acid-precipitable material in 10 minutes at 37°C, using poly(rA)-oligo dT as a template:primer.
- Heat Inactivation: 65°C for 20 minutes
- Quality Control: Enyme tested for its ability to synthesize full length cDNAs from RNA templates. Purified free of detectable levels of RNase, endonuclease and exonulease. Incubation of 100 units of enzyme for 2 hours at 37°C in 50 jal of reaction buffer with 1 jag template RNA resulted in no detectable degradation of the RNA determined by gel electrophoresis. Incubation of 100 units of enzyme with 1 ug of plasmid DNA in 50 jil of reaction buffer at 37°C for 16 hours resulted in no detectable degradation of DNA as determined by gel electrophoresis.
- Expiration: Twelve months from shipment date
- Storage and Handling: -20°C