Model 1002-G -rgp120 HIV-1 IIIB Conjugated to Colloidal Gold
rgp120 HIV-1 IIIB conjugated to Colloidal Gold (25nm mean particle size). Concentration: See vial, Volume/vial: 1ml Gold Conjugate, Diluent: 10mM Tris, pH 8; 5% BSA; 0.05 PEG; 0.02% NaN3 , Storage: 4°C, Stability: At least 3 months at 4°C Minimum, Dilution: 1 : 50, Application: Dot Blot Assay-CD4 Binding, Dot Blot Assay-Anti-gp120 Antibody Binding, Immunohistochemistry.
Coupling Ratio: OD 520nm = 10; protein content approximately 25µg.
Specificity: Gold conjugated rgp120 binds to murine monoclonal antibodies of defined epitope specificity and human polyclonal antibodies as determined by dot blot assays.
Biological Activity: Not determined.
Application and Instructions for use:
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
1. Dot blot assays may be performed in 1 : 100 dilution range depending on the concentration of solid phase CD4/Anti-gp120 in the test system.
2. Immunohistochemical studies may be performed in 1 : 50 dilution range