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Model KIR3DP1 (NM_001015070) -Human Over-Expression Lysate

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Lysate Of Over-Expression Cell Lines, WB Control. Transient overexpression lysate of killer-cell Ig-like receptor (KIR3DP1).

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Product Name

KIR3DP1 (NM_001015070) Human Over-expression Lysate

SKU/Catalog Number

LS768329

Size

100 µg

Description

Transient overexpression lysate of killer-cell Ig-like receptor (KIR3DP1)

Storage & Handling

Cite This Product

KIR3DP1 (NM_001015070) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS768329)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.