Model LC-A-24 -Hydrophilic Interaction Amide Cartridges

Application: Glycopeptide enrichment following protease digestion can be achieved using LudgerClean A Cartridges, enhancing your UHPLC and/or MS signals. Glycopeptides signals are attenuated in a complex mixture, as they are suppressed by non-glycosylated peptides. Enrichment can be performed before or after a labeling reaction. The cartridges contain a unique solid phase extraction (SPE) resin that binds a wide range of glycopeptides and allows the enrichment of these from peptide/glycopeptide mixtures. They are compatible with many leading vacuum manifold SPE handling systems.

Description: For enrichment of glycopeptides after protease digestion (eg. Sequence grade trypsin, pronase). Suitable for enrichment/cleanup after glycopeptide labeling with AQC (6-aminoquinolyl-N-hydroxy-succinimidyl carbamate) and V-tag.

Binding Capacity: Each cartridge can typically bind glycopeptides released from up to 100 μg of glycoprotein.

Number Of Samples: LudgerClean™ A cartridges are designed for single use only.

Suitable Samples: A wide range of glycopeptides can be purified. This includes the purification of glycopeptides that have been generated from different proteases, like trypsin or pronase.

Storage: Store at room temperature in the dark. Protect from sources of heat, light, and moisture. The cartridges are stable for at least two years as supplied.

Shipping: The product can be shipped at ambient temperature.

Handling: Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrate.

Safety: Please read the Material Safety Data Sheets (MSDS`s) for all chemicals used. All processes involving hazardous reagents should be performed using appropriate personal safety protection - eyeglasses, chemically resistant gloves (e.g. nitrile), etc. - and where appropriate in a laboratory fume cupboard
For research use only. Not for human or drug use

Additional Reagents and Equipment RequiredReagents

• Pure water (HPLC grade)
• Acetonitrile (HPLC grade)
• Trifluoroacetic Acid

• Manifold Cartridge Holder Plate (LP-HOLDER-96)
• Plug Pack, 12 strips of 8 plugs, to seal empty cartridges in preparation for vacuum (LP-PLUG-96)
• Deep-well collection plate (Collection Plate Pack, LP-COLLPLATE-2ML-96)
• Pipettes
• Vacuum manifold