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Sierra BioSystems, Inc.
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Model Shasta 96/192 - Oligo Synthesizer

Model Shasta 96/192 -Oligo Synthesizer

FromSierra BioSystems, Inc.

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Our patented design allows the user to drain selective rows or columns at a time, with no vacuum system needed to drain the plates or columns, providing for a faster, more cost effective, consistent and stable environment throughout the run. This selective draining feature allows the user to run 16 individual protocols in parallel, massively increasing efficiencies. Optimization of synthesis protocols can be performed in a single run, testing up to 16 different conditions without having to worry about run-to-run variability. The use of positive pressure to purge isolated pathways means it is no longer necessary to dispense ACN to each unused well, there is no longer a need to stop to allow the entire plate to drain, and the operator can select as few as 6 columns to drain at a time in the 96 well configuration, or a single row in the 192 configuration, for greater efficiencies, more consistent and faster run times, and higher yields.

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With access to 16 dedicated drain pathways, the user can now engage 16 protocols simultaneously within one run. This means 6 columns for each of the 16 conditions in the 96 well configuration, or one row for each condition in 192 configuration. All amidite and reagent handling can be made specific to each protocol. This includes injection order, injection volume, reaction time, and Pre/Post-synthesis handling procedures.

Multiple scales can be run simultaneously on the same plate. Any user-defined range is allowable. The instrument can accommodate any quantity of support that will fit within a standard high-throughput synthesis column.

The Shasta has been engineered with an understanding of the highly reactive chemistry necessary to synthesize oligos. Every piece with direct exposure to fluids or vapors is made from and/or reinforced with PTFE, Kalrez®, PEEK, or Stainless Steel. All fluidic and electronic pathways are shielded from the user. Parts subject to replacement are made easily accessible to minimize down-time.

Built to UL’s Standards, the Shasta has passed all testing to achieve compliance with UL’s current laboratory safety regulations, and it is the first oligo synthesizer in over 20 years to do so. All Shasta instrumentation is regularly inspected and approved by UL’s regulatory commissioners.

As with all Sierra BioSystems products, the Shasta comes with customer-site installation, a software and synthesis training package, and a one-year warranty, which includes parts and labor. Full Factory Service and Preventative Maintenance contracts are available.

FEATURES

Standard offerings on Shasta
instruments include:

24 Amidite Positions configured for Septum Vials or Boston Rounds per customer specification
8 Reagent Positions configured to accept 28/400, GL38, or GL40 threads
End-to-end Kalrez® and PTFE seals on every delivery line
2 – 40nmol synthesis in typical 384 well configuration
2 – 1000nmol synthesis in typical 96 column configuration
Amidite-Specific handling
Multi-Protocol Map™ Synthesis
Sulfurizer integration
Positive pressure reaction chamber ensures anhydrous environment
Full software – no license fees
One year warranty and spare parts package

CONSUMABLES

The Shasta is designed to work with
readily available consumables:

ABI and Biosearch style columns
Loose CPG and Fritted columns
Polystyrene loaded columns
Seahorse 96 well plates
Biocomma 96 well plates