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SBI System Biosciences, LLC
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Model SmartSEC -Mini EV Isolation System

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Leverage powerful SmartSEC™ technology in a spin column format—isolate EVs from small volumes of biofluids from serum to Developmental Biology samples. The only commercially available EV isolation kit for sample volumes as low as 10 µL, Great for isolating EVs from a range of biofluids, Validated in samples from human as well as model developmental biology organisms, Delivers better purity and yield than ultracentrifugation, Compatible with most downstream applications

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Explore new frontiers in EV biology

Whether it’s precious clinical samples or hard-to-collect biofluids from developmental biology model organisms, researchers are increasingly challenged by the need to isolate EVs from quite limited sample volumes. To support these researchers, SBI has configured our powerful SmartSEC™ technology into the first commercially available kit optimized for isolating EVs from 10 – 100 µL of biofluid (Table 1). Validated for samples from Drosophila, Planaria, and Arabidopsis, as well as human, mouse, and rat, the SmartSEC™ Mini EV Isolation System is ready to help you push the boundaries of EV biology.


SmartSEC Mini is a proprietary chromatography-based EV isolation technology that combines all the benefits of size exclusion chromatography (SEC)—purity, yield, reproducibility, and preservation of EV integrity—with a contaminant trapping feature that overcomes the limitations of conventional SEC for fast, easy, and low sample-volume EV isolation.
  • The only commercially available EV isolation kit for sample volumes as low as 10 µL
  • Great for isolating EVs from a range of biofluids
  • Validated in samples from human as well as model developmental biology organisms such as:
    • Drosophila melanogaster
    • Planaria
    • Arabidopsis thaliana
    • Mouse
    • Rat
  • Delivers better purity and yield than ultracentrifugation
  • Compatible with most downstream applications such as mass spectrometry, western blotting, nanoparticle tracking analysis (NTA), and transmission electron microscopy (TEM)
Each SmartSEC Mini kit comes with pre-loaded SmartSEC Mini columns, collection tubes, and sufficient column buffer for processing 10 samples (see Table 1 for loading volumes).

The SmartSEC Mini workflow is quick and easy (Figure 1). Simply pre-wash the SmartSEC Mini column, load your sample, incubate at room temperature for 30-minutes, and centrifuge for 30-seconds at 500 x g to recover isolated EVs. To maximize sample recovery, simply add more buffer to the column and collect up to two additional fractions.