Model TempoATP -ATP Biosensor Assay
TempoATP™, a fluorescent biosensor, is incorporated into Tempo’s proprietary immortalized human neuro-epithelial cell line, TempoRapid™. The biosensor assay offers fast kinetics (resolution is in seconds) in measuring real-time cellular ATP metabolism and cytotoxicity. Analysis of cellular oxidative phosphorylation directly reflects mitochondrial health and function.
TempoATP™-Rapid cells serve as a control for all products containing the TempoATP™ biosensor. To customize TempoRapid™ cells to scientists’ experimental needs, the cells are easily transfectable with gene(s)-of-interest.
For filter-based instruments, Excitation/Emission: 619nm/635nm (Red) or Excitation/Emission: 517nm/535nm (Green)
For monochromator-based instruments, Excitation 590nm +/- 25nm; Emission 655nm +/- 20nm.
TempoATP™ incorporated cells are intended for research and development, compound discovery and therapeutics development use only. It is not a product for human testing or diagnostics.
- Cytotoxicity Assays
- Cellular Metabolism Assays
- Chemical compound or small molecules testing
- Live cell imaging (time-course studies and drug treatments).
- For low, medium, or high-throughput applications
- Easily transfected cells to test gene-of-interest function(s).
Long-term Storage: liquid nitrogen
Growth Properties: adherent
Technology used: an in-house developed proprietary feeder-free, serum-free, viral/genetic-elements-free, integration-free reprogramming.
QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)
Product Use: TempoATP™-Rapid cells are intended for scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.
TempoATP™-Rapid SKU401