BTS - Modified Postgate’s B Media (MPB)
Detection of Sulfate Reducing Bacteria (SRB). This highly sensitive SRB growth medium is currently the NACE TMO-194 standard for the cultivation of SRB in oil and gas systems; Modified Postgate’s B (MPB) Media was formulated by SRB research pioneer John Posgate in 1975; MPB is a strictly anaerobic media and is manufactured at BTS inside custom built anaerobic chambers.
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- MPB contains nutrients such as proteins, organic acids, and sulfate specially formulated to facilitate the growth of oil and gas field SRB
- The redox potential of properly made MPB, typically less than -300mV, will allow for the reduction of the sulfate to sulfide by SRB
- The biogenic sulfide created by SRB then reacts with dissolved iron species in the MPB solution. This reaction precipitates a black iron sulfide precipitate. Black precipitation in the vial indicates a positive for SRB
If a water source inoculated into MPB has a significant concentration of dissolved sulfide, this can cause iron sulfide to form and create a false positive.