MDX - Multiferm Cell Culture Nioreactor
A new multiple cell culture bioreactor for medical and scientific pretesting and tests
"MultiFerm" is an automated and easy-to-use 12-fold fixed-bed fermenter system of the parallel cultivation of sensitive animal, human and other shear sensitive cells which are without stable cell wall. The system is used in the production of monoclonal antibodies, cultivation of adherent and recombinant cells and in the cell-physiological studies of tissue cells as example tumour cells, primary cells, stem cells, liver and kidney cells. The fixed-bed system is equipped with the necessary measurement and control facilities, probes and sensors, peristaltic pumps, gas supply, control program and computer for saving, evaluation and transfer of the data to other computers. The system consists of an outer 1.5 l media conditioning vessel and 12 inner fixed bed reactors which are made of Duran glass and are fitted in the middle of the media conditioning vessel. The conditioning vessel is made of Duran glass also. This makes it possible to watch, to check and to control the development of the cell cultivation. The lid, the in and out leads are made of insensitive PEEK material to protect the sensitive cells from damages by free metal ions by the usual metal lids. The fixed-bed material consists of highly porous glass sinter pearls or other suitable carrier materials and is inside the fixed-bed reactors. The cells are transferred to the surface of the carrier material in the fixed-bed reactors and grow and increase on, around and between the carrier materials and can be used for further tests and examinations.
Depending on cell-types and growth conditions "MultiFerm" is best suited for the parallel cultivation of the same or different cell lines which grow under the same or almost the same growth-conditions.
The fermenter system is excellently suited for pre-testing and testing of medicine, biotechnology, pharmaceutical and food industry.
- Total volume 1.5 Litre
- Working volume 12 x 10 ml fixed-bed reactors
- Working principle Axial
- Sterilization Autoclaving
- Temperature ranges 5 – 60o C
- pH ranges 3 - 10
- Pumps for circulation of media and continuous cell cultivation
- Flow rates 12 x 0.15 – 3 ml/min
- Heating directly via heating stick
- Operation Batch, Fed-Batch, continuous
- pH regulation Acid, alkali or CO2
- Gas-additions (outer vessel) Air, O2, N2, CO2
- Gas humidification yes
- Waste gas condenser yes
- Sampling system yes
- Diving pipes yes
- Electrodes and cables yes
- Light on request
- Process control system yes
- PC interface yes
- Data acquisition yes
- Data saving yes
- Data transfer to other computers Yes