Postnova - Model AF2000 -MultiFlow Universal Separator
The most Advanced Flow FFF Platform for Separation of Proteins, Macromolecules and Nanoparticles!. The new Postnova AF2000 MultiFlow FFF Series was invented to become the first professional modular Flow FFF platform available. It is completely integrated by the NovaFFF single software platform which runs the entire system from autosampler to detectors. The AF2000 Series incorporates the combined solid know-how and the technologies from three decades of leadership in FFF. Due to its unique design, the AF2000 MultiFlow platform offers more flexibility, higher robustness and better performance than any system before. The technology sets a new standard in the area of Flow FFF and marks a real break-through in FFF science and therefore naturally has become the leading Flow FFF solution for many scientists around the world.
The AF2000 Series platform is based on the Flow FFF principle, using a crossflow field as driving force for the separation. The samples which are affected by this field are separated by their Dynamic Diffusion on the basis of the molar mass or particle size. The AF2000 can be run with different eluents, at various temperature conditions and is able to employ various channel dimensions and principles. The same system can be used for running planar Asymmetric Flow FFF (AF4) channels or circular HollowFiber Flow FFF (HF5) channels. Therefore the AF2000 MultiFlow can be easily used for separation of a broad range of different samples from the area of Biopharmaceuticals, Food-Agro-Cosmetics, Environmental, Chemicals and Nanotechnology. The MultiFlow FFF is ideal for the characterization of proteins, antibodies, aggregates, vaccines, liposomes, nanoparticles and natural or synthetic macromolecules. Because of its great flexibility, the AF2000 MultiFlow FFF can be employed as a Universal Separator for many kind of analyte systems and is a secure investment also for new upcoming samples and future project demands.
The AF2000 AT is a non-temperature controlled economical Flow FFF which is ideal for the use in general level research in universities, companies and public scientific centers. It is available as aqueous metal-free, stainless steel and multisolvent version and can be run with a broad range of aqueous and organic solvents. Planar Asymmetric Flow FFF (AF4) and circular HollowFiber Flow FFF (HF5) channels can used at the same time. Various detection principles, such as UV-Absorbance (UV), Refractive Index (RI), Multi-Angle Light Scattering (MALS), Dynamic Light Scattering (DLS) and Inductively Coupled Plasma Mass Spectroscopy (ICP-MS) can be coupled easily.
The AF2000 MT is a temperature controlled advanced Flow FFF system, which is ideal for the use in higher level research in university and public research institutions as well as professional research and commercial-industrial applications in the area of product development, manufacturing and quality control. It is also available as aqueous metal-free, stainless steel and multisolvent version and can be run with a broad range of aqueous and organic solvents. Planar Asymmetric Flow FFF (AF4) and circular HollowFiber Flow FFF (HF5) channels can used at the same time. Various detection principles, such as UV-Absorbance (UV), Refractive Index (RI), Multi-Angle Light Scattering (MALS), Dynamic Light Scattering (DLS) and Inductively Coupled Plasma Mass Spectroscopy (ICP-MS) can be coupled easily. Temperature control is not only available for the separation channel but also for the entire system, incuding the transfer capillaries between the various modules and detectors.
The AF2000 HT is a special temperature controlled toplevel Flow FFF system, which is ideal for the use in research, development and quality control of polyolefins and high temperature macromolecules in universities, public research institutions and industry. It is available as stainless steel version and can be run with organic solvents from ambient temperature up to high temperature conditions. Special planar Asymmetric Flow FFF (AF4) channels made from stainless steel and ceramics are exclusively employed for this conditions. As detection principles Infrared (IR), Refractive Index (RI), Multi-Angle Light Scattering (MALS) and Viscometry (Visco) can be used.
In order to enable the user to fully access this wide application range, the AF2000 MultiFlow is available in different unique versions. Various add-on module, extra options and special FFF detectors are available, such as Refractive Index (RI), Multi-Angle Light Scattering (MALS) and Dynamic Light Scattering (DLS). All Postnova FFF modules and detectors have been especially developed for FFF and are perfectly optimized not only for the use with the AF2000 MultiFlow FFF, but also for the other Postnova FFF versions. Aditionally the AF2000 Series has been designed for easy interfacing with existing high-end detection technologies from leading instrument suppliers, so that the user can ideally integrate the Postnova FFF technology into the existing laboratory infrastructure in order to maximize efficiency and investment.
AF2000 AT - Ambient Temperature Flow FFF
The most flexible, robust and reproducible Entry-Level Flow FFF system for Research Applications.
Application Range
The AF2000 AT is the basic modular system which marks the Entry Level of the Postnova AF2000 MultiFlow platform. The system is non-temperature controlled and runs at typical ambient laboratory conditions from 15° to 25°C. It is ideal for standard research applications in university research groups and educational institutions with a cear focus and defined application range. The AF2000 is the best choice and a good starting point for groups which want to enter into the area of Field-Flow Fractionation for the first time. It is available as aqueous metal-free, stainless steel and multisolvent version and can be run with a broad range of aqueous and organic solvents. Planar Asymmetric Flow FFF (AF4) and circular HollowFiber Flow FFF (HF5) channels can used at the same time. Various detection principles, such as UV-Absorbance (UV), Refractive Index (RI), Multi-Angle Light Scattering (MALS), Dynamic Light Scattering (DLS) and Inductively Coupled Plasma Mass Spectroscopy (ICP-MS) can be coupled easily online.
System Hardware
It is the Postnova`s clear mission to only offer fully optimized and professional FFF hardware, software and support solutions. Therefore the AF2000 AT series is a basic but completely optimized Flow FFF system, which contains all neccessary standard modules and components in the package, which are required for a robust, reliable and fully operable Flow FFF system. There is no need for assembling and utilizing semi-suited third party HPLC components, which are often just made for other purposes, show less flexibility and are not avalibale in the strict specifications needed for FFF applications. The AF2000 AT series is a true ready-to-use non-compromise Flow FFF system, where no additional components are required.
The system incorporates the compete set of Postnova`s patented and proven FFF technologies, such as FocusTech, ChannelCartridge and SyringeXFlow, which make it the most easy-to-use, robust and reproducible system available. The AF2000 AT system is comprising an Eluent Organizer (PN7205), an Eluent Degasser (PN7520), an Eluent Pump (PN1130), a Focus Pump (PN1130), a Flow FFF Control Modul with integrated electronic interface and cross-flow syringe pump (AF2000 Modul), a Manual Injector Holder (PN5120), a user-selectable Asymmetric / HollowFiber Flow FFF channel (AF4/HF5) and a Control PC with the NovaFFF software suite for operation of the AF2000 AT system.
Additional System Options
The AF2000 AT Flow FFF is available in different versions and additionally can be upgraded and customized for special applications by adding extra system options. This can be done at date of purchase or later as an upgrade.
- EFS Eluent Filtration System
- UVD Eluent Disinfection Module
- SAM Manual/Auto Injector with Options
- ELC Aqu. Metal-free/Organic/MultiSolvent
- AF4/HF5 Channel Versions and Membranes
- S3 Outlet Splitting Sensitivity Enhancer
- RID Refactive Index Detection System
- UVD Absorbance Detection System
- FLD Fluorescence Detection System
- MALS Multi-Angle Light Scattering Detector
- DLS Zetasizer* Dynamic Light Scattering Detector
- ICP-MS Online Connection and Interface
- FRA Automatic Fraction Collector with Options
System Software
The unique NovaFFF single software platform is used for full control and data acquisition of the entire AF2000 AT Flow FFF together with associated peripheric modules (such as Auto Injector, Fraction Collectors) and detectors (LS, RI, UV, FLD). The software also allows comprehesive data analysis and evaluation based on Relative Standard Calibration and Absolute Light Scattering methods. Furthermore the software is also the platform to run all mayor FFF versions, including AF2000 MultiFlow FFF, CF2000 Centrifugal FFF and TF2000 Thermal FFF. This together with additional features, makes NovaFFF the most advanced and leading software platform available for FFF applications today.
Service & Support
In order to assist our users in achieving the best results and getting the maxium output from their FFF system without any time delay, Postnova offers a series of differently adjusted services and support activities. These services have been optimized to perfectly fit each step the live cycle of any FFF system, from installation and initial training, via maintenance during operation all the way to relocation and upgarde services to keep up with new technological developments.
- Installation Services
- User Training Courses
- System Maintenance
- Relocation Services
- Upgrade & Update Services
Broad Separation Range
Other than as in HTGPC, the separation in HTFFF can also be performed for polymers up to molar masses of 10E9 Da. No size exclusion effect is observed, making HTFFF the ideal tool for the separation of large and ultra-large polymers or even nanoparticles.
Gentle Separation Conditions
Because of the open channel and the absence of any stationary phase the separation can be performed under the absence of shear forces and stress to the macromolecule. Typical HTGPC problems, such as filtering/adsorption effects, shear induced chain degradation and late elution effects can be avoided.
Patented FOCUS Technology
The AF2000 HT incorporates Postnova’s patented FOCUS technology, to ensure a continuous and stable detector flow at any time. This is an absolute necessity, to be able to connect flow sensitive Viscometers and RI detectors. Additionally to this, the technology provides also a higher sample recovery, higher resolution and sensitivity. Another striking advantage of the Focus technology is that the channel outlet can be directly connected to the detector without any valves or T-pieces build in between. This ensures short transfer line, low dead volumes and minimized band broadening.
Patented Cross Flow System
Postnova is using a patented technology for generating the cross flow inside the FFF channel, based on a double syringe pump. This pump is controlled in a special unique way avoiding any pulsation. The pump is completely inert and can easily produce constant and absolute flows ranging from 0,005 to 10 ml/min for different solvents. Using the volumetric based syringe pump technology, a certain volume per time is removed independent from the nature of the solvent and the temperature.
Flexibility & Interfaceability
The AF2000 MT series was developed to be used as Stand-Alone system or interfaced with existing HTGPC systems from various manufacturers. This gives the advantage that FFF and GPC can be performed using the same set or detectors, using the same temperature chamber for GPC and FFF.
The NovaFFF software is based on a “one Software for all devices” strategy, incorporating system control, data collection and data evaluation as well as reporting.
The easy to use NovaFFF Software:
- Very flexible: simple integration of hardware components e.g. autosampler, pumps and various detectors
- Important security and monitoring functions: pump and channel pressure, manually or automatically shut down of the system when a set maximum pressure value is exceeded.
- Run data file: pressures, flows and additional analog detector signals are stored
- log history file: main actions and controls are logged
- PC and LAN controlled: integration within the companies local network (LAN) with own IP address for every FFF system. State-of-the-art communication protocols using TCP/IP
- unique FFF-LS Integration: controlling the FFF platform together with Light Scattering detector in just one application.
No more start-up of two programs, copy and pasting of methods between different programs or problems about the stability of the multitasking capabilities of the PC operating system when using NovaFFF.
Temperature Range:
- Cooling real 5°C
- Heating max. 80°C
Measurement Range:
- Polymers: 104-109Da / Nanoparticles: 1nm-10µm (Depending on membrane and sample material)
Channel Cartridge System:
Patented Postnova channel system:
- Inner Volume: 0,5 -1,5 mL depending on spacer
- Inner Channel Thickness: 350 µm (others available)
- Outer Dimensions: 335 x 60 x 40 mm
Applicable Solvent Systems:
All typical organic and aqueous solvents.
Connected Detector Systems:
Typical LC and most other well-known detectors can also be used in AF4:
- Low/Right/Multi Angle Light Scattering for MW/Rg
- Viscometric Detection for MW/Rh
- Refractive Index for Concentration
- Infrared Detection for Polyolefin Concentration
- Other Technologies: Please inquire on availability
Flow Rate Ranges / Pressure:
- Detector Flow 0,01-2,0 mL/min at channel outlet
- Cross Flow 0 or 0,005-10 mL/min
- Channel Pressure up to 50 bar maximum
Required Analysis Time:
Typically 10-20 min; shorter/longer times possible
Electrical Power Requirements:
- 230V, 50Hz: Min-Max / 1,2 kW-3,6kW at 6,3-15,6A
- 110V, 60Hz: Min-Max / 0,6 kW-1,8kW at 6,3-15,6A
- 100V (Japan): Please inquire for power requirements.
- All values are for the basic set-up without detectors only.
PC Requirements:
Windows OS, min.1024 MB RAM, 2 Ethernet ports
Software System:
Unique NovaFFF software platform for complete system control (autosampler, pumps, detectors), data acquisition (detector signals, pressure, flows) and evaluation as well as reporting functions (static light scattering calculations, standard calibration).
Maintenance Parts:
Pump seals, pistons and check valves as usual. The channel cartridge itself is inert and made of special unique materials. It can be regenerated after heavy use and thus be re-used many times.
Injection Volume:
- Typically 10-1000 µL with autosampler injection
- Typically 10-10.000 µL with manual injection
Band broadening is not affected by injection volume because of patented focus technology used inside.
Injected Sample Mass:
Limiting parameter is channel overloading, which is primarily depending on the channel dimensions. Typical injected sample mass for given channel size is 10-100 µg. The maximum sample mass can go up to 500 µg, depending on sample nature.