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NanoCuvette - Model One -Laboratory Workflow for Quantitative Analysis
The NanoCuvette™ One is an excellent improvement in any laboratory workflow for quantitative analysis in numerous industries such as biotechnology, pharmaceuticals, chemistry, foods and industrial applications for quality control. The capacity of NanoCuvette™ One goes far beyond any product currently on the market in terms of concentration determination. With the NanoCuvette™ One you are able to analyze smaller samples (down to 0.5 μL) and a large range of concentrations (0.02 - 300 mg/mL) of non-absorbing compounds.
The NanoCuvette™ One expands the capabilities of your spectrophotometer. It allows you to perform classic absorbance (attenuance) spectroscopy and label-free spectroscopy via refractive index. Integrated in the surface is a patented photonic nanocrystal (optical filter), which bends the light beam such that it senses refractive index near the photonic crystal surface in the wavelength range from 550 nm to 800 nm.
Traditionally, spectrophotometry laboratory work has been limited by lack of robust methods to determine concentrations. We upgrade UV-Vis spectrophotometry around the world and across all major instrument brands to create new ways to determine concentration, perform enzymatic studies in a label-free, fast, cheap, and reliable method.
Absorption versus label-free spectroscopy
With absorption spectroscopy, the Beer–Lambert law relates the attenuation of light to the properties of the material through which the light is traveling:
where A is the absorbance, ε is molar extinction coefficient, c is the concentration, l is the path length and A0 is the background absorbance.
In contrast, when light hits a nanoscale photonic crystal, the resonance wavelength is related to the refractive index or concentration close to the surface:
where λ is the wavelength, β is a coefficient, α is the specific refractive increment, c is the concentration, ns is the solvent refractive index and λ0 is the reference resonance wavelength, which is derived from Snell`s law and this equation is called Hand`s Law. In this application this is called label-free spectroscopy
Lift the standard of lab analysis
- Measurements at different volumes (0.5 μl, 3 mL) and concentrations (0.02 - 300 mg/mL)
- Non-absorbing protein concentrations
- Monitoring label-free enzymatic reactions
- Monitoring non-absorbing/non-fluorescent kinetics
- QC measurements
Enzymes and proteins can be expensive, some equipment needs 2 to 3 μL to measure protein. The NanoCuvette™ One can measure volumes down to 0.5 μL and concentrations down to 0.2 mg/mL for sucrose (Limit of detection is below 0.2 mg/mL sucrose concentration).
NanoCuvette™ One’s optical filter measure refractive index and together with SpectroWorks™ it will calculate the refractive index similar to SPR. Refractive index does not require any coloration (dye or indicator) to be measured. Thus it is possible to measure concentrations with spectrophotometer of a transparent solution.
Direct label-free detection of enzymatic activity with a substrate can be monitored with the change in the intrinsic property refractive index of the solution as product is formed over time. The NanoCuvette™ One has a built-in optical filter allowing it to measure refractive index changes due to enzymatic reaction kinetics in real time using an UV-Vis spectrophotometer.
Supports Your Hardware
NanoCuvette™ One likely works with your existing UV-Vis spectrophotometer! Read more here.
Designed For Humans
Easy to use with no special training required. Online support included.
Accurate and Instant Results
Results are calculated based on spectral analysis. Our engine in SpectroWorks™ is fast and makes it a breeze to extract results.
Unique Traceability
First in the industry, each cuvette is marked for traceability and access to online calibration data.
Semi-reusable cuvettes
The NanoCuvettes™ can be re-used up to a certain amount depending on application and maintenance.
Less sample needed for analysisMeasurements at different volumes (0.5 μl, 3 mL) and concentrations (0.02 - 300 mg/mL)