NanoFCM - Flow NanoAnalyzer

The Flow NanoAnalyzer paves a way for the mutiparameter analysis of the nanoparticles from 7 to 1000 nm with its unprecedented sentivity in scatterring and fluorescence detection. The Flow NanoAnalyzer delivers high-resolution data at the single-particle level to dicipher the subpopulations and compositions of various nanoparticles, such as extracellular vesicles, LNPs and virial vectors. 

Superior Sensitivity
Scattering detection can be down to single gold and silica NPs as small as 7 nm and 24 nm, respectively.

Real-Time Traces
The real-time burst traces are provided to monitor the status of smaples and ensure the particles are detected individually.

Size and Concentration
Particle size distribution and concentration can be acquired in minutes without labels. The size results with a resolution and profile match well with those of TEM measurements.

Phenotyping
Combined with fluorescent labedling, multiple subpopulaiton for nanoparticles with biomarkers of low aboundance are identified.

Subpopulation, particle size, concentration, and function obtained from a single test

• Size range: 7-1000 nm
• Single / Multidisperse systems
• The results reported for 2-3 min
• 10 μL sample as low as 1 μL of consumption
• The resolution is comparable to the frozen TEM
• Both Label free and labeled samples are applicable
• The scattering was as low as 24 nm and the fluorescence aslow as single PE detection
• The suspension status, keep the sample native state detection

Superior Sensitivity in SS and FL Mearsurement

Employing strategies for single-molecule fluorescence detection in a sheathed flow, Flow NanoAnalyzer enables light scattering detection of single gold and silica nanoparticles as small as 7 and 24 nm in diameter, respectively. For fluorescent detection, single phycoerythrin can be distinguished from the background.

Real-Time Monitor

The real-time burst traces provided the original signals of particles of interest and the background state. These can be used to monitor the status of smaples and prevent swarming detection. 

Particle Size Distribution and Concentration Measurement

Based on light scattering measurement of individual nanoparticles, accurate and statistically representative particle size distribution can be acquired in just minutes with a resolution comparable to that of TEM or cryo-TEM. Furthermore, particle concentration can be determined by using single-particle enumeration at the same time. 

Phenotyping for Subpopulation Identification

Upon fluorescent staining, Flow NanoAnalyzer acheives the analysis of compositions including lipid membrane, nucleic acids and specific proteins of extracellular vesicles, LNPs and viral vectors. This enables scientists to create an objective benchmark to  dicipher the heterogenous subpopulations of nanoparticles.

Extracellular Vesicles

Flow NanoAnalyzer achieves the sizing, concentration measurement, and subpopulation identification of EVs down to 40 nm with a throughtput up to 10,000 events per minute. 

mRNA LNPs
Flow NanoAnalyzer effortlessly achieves the resolution of various components within a single sample, including nucleic acid-loaded LNPs, empty LNPs, and free nucleic acids. This provides a brand-new solution for the loading ratio determination, and the qutification of particle size distribution and concentration of each fraction.

Lentiviriuses
By integrating fluorescent labeling, Flow NanoAnalyzer enables the identification and quantification of various components within lentiviral preparations, such as VSV-G, nucleic acids, and P24. This facilitates the precise measurement of intact virions, partially packaged viruses, and impurity particles. Such capability offers a significant advantage in the preparation and quality control of lentiviral vectors.

Adenoviriuses (ADVs)
For adenoviruses, Auto can distinguish between intact and empty capsids solely through scattered light detection without labels.