Nicoya Alto - Nanotechnology-based Digital SPR System for Precise Biomolecular Analysis
The Alto Digital Benchtop SPR System, introduced by Nicoya Lifesciences, marks a significant advancement in the field of surface plasmon resonance (SPR) technology. It is the first SPR instrument to combine digital microfluidics with nanotechnology-based biosensors, revolutionizing fluidics interactions through disposable, integrated microwell plates. Alto's design enables precise management of 2µL sample volumes while accommodating crude samples without requiring fluidics maintenance. Highly compatible with automated processes for both hardware and software, Alto provides efficient sample-in/answer-out data with leading throughput and offers over 24 hours of autonomous operation. Its user-centric design incorporates a flexible 16-channel setup and an intuitive data analysis platform to enhance ease of use. The system supports applications such as kinetics and affinity characterization, quantitation, and epitope mapping, and works with a wide range of sample types, including proteins, nucleic acids, viruses, and small molecules. Additionally, the integrated Nicosystem software streamlines data acquisition and analysis, promoting accessibility and remote collaboration.
Alto is the world’s first SPR instrument to integrate digital microfluidics (DMF) with nanotechnology-based biosensors.
DMF technology enables all fluidics to be disposable, allowing for precise handling of 2µL sample volumes, crude sample compatibility and zero fluidics maintenance. Alto’s compatibility with automation for both hardware and software provides sample-in/answer-out data, industry-leading throughput, and 24+ hours of unattended run time.
Every aspect of Alto is designed with user-friendliness and accessibility in mind-from the flexible 16 channel design to the intuitive data analysis platform.
The first ever DMF-powered SPR system
Alto cartridges have completely revolutionized the interaction of fluidics and SPR sensors by integrating them into one disposable microwell plate.
Applications
- Kinetics/Affinity Characterization
- Kinetics/Affinity Screening
- Quantitation
- Epitope Mapping/Binning
Compatible With
- Proteins / Peptides
- Antibodies
- Nucleic Acids
- Carbohydrates
- Crude samples
- Viruses
- Small Molecules (application dependent)
Surface Chemistries
- Carboxyl
- Anti-His (kit)
- Streptavidin (kit)
- Protein A (kit)
- Decoupling of flow rate and sensor position from dispersion, increasing data quality
- Up to 200X less sample volume required – get full kinetics from a 2µL sample volume
- Decoupling of interaction time from sample volume, increasing assay flexibility
- Automated serial dilutions – reducing human error and saving time
Performance
- Association Rate (kon) : Up to 1x10⁹ 1/M*s
- Dissociation Rate (koff) : 1x10⁻⁵–1.0 1/s
- Affinity Range (KD) : pM - mM
Hardware
- Core Detection Technology : Localized SPR fiber optic biosensors
- Data Channels : 16
- Analytes : Up to 48 analytes/cartridge
- Sample Volume : 2μL
- Reference:Standard : 1:1
- Dilutions : Automated serial dilutions
- Unattended Run Time : 24+ hours
- Compliance : Please inquire for your specific needs
- Instrument Size : 1.5H x 1W x 1.5D (ft)
- Instrument Weight : 51 lbs