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Navigo Proteins GmbH
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Navigo - Model Precision X Platform -Recombinant Protein Purification Technology

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Advantages of Artificial Protein A Scaffolds: Precision X affinity ligands are small (~6.5 kDa), selective and stable proteins based on our proprietary artificial Protein A scaffold. They no longer have to bind to antibodies’ Fc parts, but instead we engineer new, specific binding characteristics. We also offer adjustable affinities and pH elution profiles, while preventing off-target binding. Our Precision X ligands and matrices possess exceptional binding capacities, high caustic and chemical stability to withstand harsh cleaning-in-place procedures in dozens of sequential downstream processing cycles, for low cost of goods. Finally, our affinity resins are amenable to generic Protein A leaching assays and can be flexibly used in analytical applications as well.
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Precision Capturing® Advantages:

  • Selective binding to targets from all biological product classes
  • High binding capacity
  • High chemical and proteolytic stability
  • No internal Cysteines for site-specific matrix-coupling
  • Adjustable mild elution conditions
  • Amenable to generic Protein A leaching assays
  • Ligands also usable for analytical applications
We have implemented and over years continuously optimized, a powerful scaffold technology platform which yields high-quality Precision X ligands against the target of choice.
Libraries

High quality and diverse libraries of artificial Protein A are generated by randomization of carefully selected surface-exposed residues. Each randomized position can comprise all natural amino acids (except Cysteine) which leads to large combinatorial libraries with complexities of 1010 to 1012 different variants each. These high-quality libraries are constantly expanded and are the crucial starting point for ligand discovery programs.

Selection and Screening

During the subsequent selection process, both phage display and ribosome display technologies are deployed to yield ligand-enriched selection pools. Out of these, our fully automated high-throughput screening (HTS) platform then identifies the desired Precision X ligands by rapid screening of up to 15.000 binders per day. The most promising leads are then selected for further development.

Specifically, the affinity ligands are coupled to a solid chromatography support (e.g. beads) to generate the corresponding custom affinity matrices (resins, membranes, etc.). These are then rigorously tested for their performance in biologics downstream processing. Following Precision X ligand and matrix scale-up and validation, the clients’ custom affinity chromatography solution is ready for clinical and commercial non-Fc biologics manufacturing.

High quality libraries are the key to our success.