New England Biolabs
NEB - Model BspQI-HF -Restriction Endonucleases
We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes.
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High-Fidelity (HF®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (rCutSmart™ Buffer). All HF-restriction enzymes come with Gel Loading Dye, Purple (6X). Enjoy the enhanced performance and added value of our engineered enzymes at the same price as the native enzyme:
- Engineered for improved performance
- 100% activity in rCutSmart Buffer
- Time-Saver™ qualified for digestion in 5-15 minutes
- Reduced star activity
- Supplied with 1 vial of Gel Loading Dye, Purple (6X)
- Linearize plasmid template before in vitro transcription
- Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
- Used in Golden Gate Assembly
- Can be used with NEBridge® Ligase Master Mix for Golden Gate Assembly
- Restriction Enzyme Cut Site: GCTCTTC(1/4)