Nexcelom - Model Celigo -Image Cytometer

Perform best-in-class brightfield imaging in combination with four fluorescence channels to provide high speed, fully automated imaging and quantification of suspension AND adherent cells, tumor spheroids, iPSC, and cancer stem cell colonies.

Proprietary optics and scanning system enable fast imaging of the entire well while maintaining consistent illumination and contrast out to the well edge, for accurate identification of all cells within each well.

With an intuitive software interface and optional integration into automation platforms, the Celigo provides labs with increased capabilities. Celigo is valuable at every step from enabling high-throughput screens of drug libraries in early drug discovery or running high-throughput validated assays for late-stage research.

Excellent Optics for Enhanced Image Quality

Improves brightfield optical image quality at the edge of wells and eliminates edge optical distortion by using an F-Theta lens for superior well edge-to-edge image contrast.

Accurately Quantify Cells and Colonies

Accurately quantify every cell and colony in the well even if they do not grow uniformly across a well.

Brightfield and Multi-Channel Fluorescence Imaging

Use a combination of brightfield and fluorescent imaging allowing the development of multicolor assays ranging from UV to Far Red.

Clear Visualization of Image and Data Correlation

Correlate the cell populations identified in gates with the cells visualized in the image using color-coding overlays.

Simple At-a-Glance Plate-Based Data Review and Growth Curves

Quickly identify cells from well to well, easily evaluate data, and generate time course growth curves to be easily displayed in a plate overview for at-a-glance data review for each plate.

Easy Brightfield and Fluorescent Image Navigation

Navigate between plates, wells, and time points to look at your cells growing in specific well locations.

Application Programming Interface (API) and Data Management Solution for Integration into Automated Workflows

Integrate with Robotics

Automate your assays by running the Celigo under the control of scheduling software and integrate with robotic arms, plate stackers, automated incubators, and liquid handlers.

Automated System for High-throughput Integrated Data Acquisition and Analysis

Acquire images and data analysis of hundreds of plates 24/7 automatically.

Time-saving Data Processing Method

Get data on the fly by acquiring and analyzing images simultaneously.

Flexible Data Analysis Method for Multiple Users

Analyze large data sets off the Celigo instrument using the Celigo Satellite Workstation and free up Celigo time to acquire more plates.

Label-Free Brightfield Cell Analysis

Take advantage of label-free brightfield applications to avoid staining cells with toxic dyes or transfecting with fluorescent reporters.

Easily Access and Manage Data using Celigo Network Database

Facilitate access to your imaging data using the Celigo network database and connect multiple instruments and satellite workstations to seamlessly acquire and analyze your data from a convenient centralized location.

Easy-to-Use Preset Assay Parameters for Quick Data Analysis

Perform Celigo assays using pre-defined parameters that require very few modifications from the researchers.

Simple and Intuitive Software User Interface

Benefit from a simple and intuitive four-step software workflow to run all your assays.

Celigo Express offers a 21 CFR Part 11 module that is compliant with the Code of Federal Regulations (CFR) Title 21 Part 11 − Electronic Records: Electronic Signatures published by the U. S. Food and Drug Administration (FDA). This module ensures that an organization’s use of electronic records and digital signatures in place of traditional paper-based documentation complies with current FDA regulations.

The newly developed Celigo Express software works with the 21 CFR Part 11 ready module to simplify the data acquisition process by allowing researchers to use projects imported from Celigo Pro (v5.4) and quickly run them to collect sample data. With a few clicks of the mouse, run a scan using Celigo Express:

• Select the project to run
• Click on Start Scan
• View your data

Key elements of the 21 CFR Part 11 module are:

• Electronic Signatures are captured during the counting and analysis workflow using e-Sign/e-Unsign buttons and include the name of an authorized user, the date/time when the signature was executed, and the meaning of the signature (i.e., indicating either Approval or Rejection of an action).
• An Audit Trail assures the integrity of an electronic record and continually monitors all users performing actions, the type of actions being performed, and the date/time associated with user actions.
• User Roles allows for specialized access control to the varying levels of Celigo Express functionality to specified groups. When creating a role, subsets of privileges can be selected to apply to users performing common or specialized tasks.
• A Close-looped Database System ensures the integrity of the data generated.

Understanding User Role Privileges

• Data − Controls whether users can create, delete, export/import, and approve/reject data during the counting and analysis workflow.
• Settings − Controls whether users can access the Projects screen functionality available under the Manage tab to allow them to update, delete, and import/export projects.
• Users, Roles, or Audits − Controls whether users can access the 21 CFR Part 11 module Users, Roles, or Audits options under the Manage tab to allow them to create, update, and delete Celigo Express users/roles, or monitor and export the audit trail automatically generated by the system after the module is enabled.

Stacker Automation: No Need to ‘Baby-Sit’ Your Plates

With the simple integration of the plate stacker, you are free to work on other tasks while Celigo screens your stack of plates.

• Up to 50 plate capacity
• 15-sec transfer rate between plates
• Data exports automatically for each plate
• Handles plates with or without lids
• Accommodates from 6-well up to 1536-well plate formats
• Fits on a standard lab bench
• Ideal for endpoint assays
• Easy to add on to existing Celigo instruments

• Proprietary image acquisition and processing software
• Powerful analysis software/Dell Precision computer
• Windows 10

Illumination/Optics

• 1 LED-based enhanced brightfield imaging channel with uniform well illumination
• 4 LED-based fluorescent channels
• Proprietary F-theta lens with superior well edge-to-edge contrast
• Galvanometric mirrors for fast imaging of large areas
• Large chip CCD camera (2024 x 2024 pixels)
• 1, 2, 4 or 8 μm/pixel resolution

• 6, 12, 24 48, 96, 384, 1536 well plates (black, white and clear wall plates)
• T-25 and T-75 flasks
• Slides and cell arrays plate profiles available upon request

High-Speed Imaging

• Less than 2 minutes per 384-well plate

Weight and Dimensions

• Dimensions: 19.5″W x 16″H x 24″D (49.5cm x 40cm x 61cm)
• Weight: 117 lbs. (53 kg)

Power Requirements

• 110-220 VAC 50-60 Hz

Regulatory Compliance

• CE marking