Nippon BlueEasy - Model MWP06 -Prestained Protein Marker
The BlueEasy Prestained Protein Ladder is a three color protein standard with 10 prestained proteins. It has the largest range of molecular weights from 6.5 to 270 kDa. The BlueEasy Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon or nitrocellulose) and for approximating the size of proteins.
The molecular weight of the marker bands can vary depending on the gel/buffer system used. This is due to factors of the gel/buffer system that affect the running behaviour of proteins, e.g. migration rate, gel composition, pH-value (see FAQ for more information).
- Broad range: 6.5 – 270 kDa
- High performance: 10 bands with 3 colours (blue, orange and green)
- Ready to use: Supplied in a loading buffer for direct loading on gels.
- Easy to identify: The 52 (green), 30 kDa and 270 kDa (orange) are reference bands.
- Sharp bands
Approximately 0.1~0.4 mg/ml of each protein in the buffer (62,5 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 10 mM Dithiothreitol, 1 mM EDTA, and 33 % (v/v) Glycerol).
500 µL Protein Marker in loading buffer are sufficient for 100 mini gels or 50 large gels.
StorageStable for up to 2 weeks at 25°C. Stable for up to 3 months at 4°C. For long term storage, store at -20°C.
Loading3 μl or 5 μL per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
- Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis.
- Monitoring of protein transfer onto membranes during Western blotting.
- Sizing of proteins on SDS-polyacrylamide gels and Western blots.