Oligo Pools - Silicon-Based DNA Synthesis Platform

Due to the sheer scale of DNA synthesis capability on the chip there are virtually no limitations to the number of unique oligos that can be assembled into a pool. Don`t restrict the outcome of your application, order Oligo Pools tailored precisely to your experiment.

More Space for Innovation
Don’t be limited by length. Encode the elements and sequences you need, including promoters, enhancers, guides, and probes all within the expanded architecture. With oligo lengths up to 300nt it’s possible to encode multiple elements in a single design giving you the ability to do more with each oligo.

Screen once, screen right
Unmatched pool uniformity without bias provides more unique targets per screen. This means less effort is required to achieve the data coverage you need, saving time and money.

Be data confident
With the precision and uniformity of Twist Oligo Pools, you can be confident in the quality of your experimental results. Don’t miss a datapoint due to synthesis errors or inefficiencies. Get industry-leading oligo representation and low error rate for oligos of any length up to 300nt, and pools of any size. Leverage these exceptional quality oligo pools at the precise scale and length required for your application.

• Oligo Length Up to 300nt
• Oligo Pool Size No limits on pool size
• Yield >0.2 fmol of each oligo
• Uniformity >90% oligos represented within
• Error rate Up to 1:2000
• Turn-Around Time Starting from 5 business days
• Price Industry-leading pricing

CRISPR screens involve the precise, high-throughput knock-out, interference or modulation of gene activity, allowing complex genotype-phenotype interactions to be unveiled. Oligo Pools are designed to encode multiple guide RNA molecules per gene. 

• Full control over guide library design, avoiding unwanted guides, wasted sequencing, and limited guide-per-gene ratios.
• Pairing high-fidelity Cas enzymes with highly accurate synthesis for true high-fidelity screens with minimal off-target effects.
• Incredibly uniform, bias-free pools achieve amplified signal to noise ratio to achieve maximum hit detection, lowering downstream costs.
• Oligos up to 300nt make space for additional experimental elements. Add multiple guides per oligo, genome homology, or minimal promoters with ease.