AmplideX - PCR/CE FMR1 Reagents

AmplideX PCR/CE FMR1 Reagents* have created an easy-to-use, accessible, high performance method for laboratories to reliably analyze CGG repeats and detect interrupting AGG sequences in the FMR1 gene.

Reduced Complexity
Ease-of-analysis of the FMR1 gene has been simplified through:

• Implementation of proprietary PCR solution for amplifying GC-rich regions
• Automation of result calling using AmplideX PCR/CE FMR1 Analysis Module

Optimized Workflow
Valuable operator hands-on time has been significantly reduced through:

• Direct injection of PCR products (no PCR clean up) in to Capillary Electrophoresis platforms
• Decreased need for Southern blot analysis (up to 50 fold)
• End-to-end solution for FMR1 analysis including all necessary reagents and software

Quality Performance
Performing FMR1 Analysis with Greater Sensitivity and Accuracy:

• Detection of all allele expansions, including low abundance full mutation size mosaics with up to at least 1300 CGG repeats
• Up to 875 fold more sensitive than Southern blot¹
• Resolution of female homozygous and heterozygous samples and indication of interrupting AGG sequences
• Proven performance as indicated by more than 30 peer reviewed publications

Analytical Characteristics of AmplideX PCR/CE FMR1 Reagents*:

• Detects all alleles including low abundance full mutations (Figure 1)
• Accurately sizes any repeat up to 200 CGG repeats (Figure 2)
• Resolves female zygosity (Figure 3)
• Detects presence of AGG interruptions (Figure 4)