IonFlux - Model 910-0054 -Plate 16 (Ensemble)

IonFlux Plates are microfluidic devices used exclusively in the IonFlux System for automated patch clamp assays. They are integrated into SBS-standard well plate formats for convenience and compatibility with automated liquid handling instrumentation. Multiple plate formats are available which correspond to the IonFlux system configuration. Each well on the plate has a dedicated function as either a compound well, cell trapping well, cell inlet or cell outlet well.

Plates are divided into experimental patterns. Each pattern contains specified wells:

• Two Independent trapping wells: These wells would contain intracellular solutions
• Cell inlet well: This well would contain the prepared cells in extracellular solution
• Cell outlet well: Acts as a waste well for the pattern
• Eight independent compound wells: These wells would contain any combination of different compound solutions or dose response concentration gradient of the same compound. IonFlux software provides full control over these compound wells release and timing.

IonFlux plates come in two different standard designs, 96 well plates for use with all IonFlux system having 16 amplifiers and 384 well plates for use with all IonFlux systems with 64 amplifiers or more.

• 96 well plate - used exclusively with IonFlux Classic 16 & IonFlux Mercury 16
• 384 well plate - used exclusively with IonFlux Mercury HT and IonFlux Mercury Ultra

IonFlux plates come in two different cell trapping and recording designs:

• Single Cells: Using whole cell patch clamp technique and achieving giga-ohm seal recordings, IonFlux single cell plates are perfect for specialized assays requiring complete voltage/current control of an independent cell, mimicking manual patch clamp conditions while providing automated systems flexibility.
• Ensemble cells: Just like single cell plates, ensemble plates also use whole cell patch clamp techniques but now with 20 cells in parallels setting a standard for consistency and throughput All IonFlux instruments can use either plate design with no hardware modifications.