- Home
- Companies
- Nippon Genetics Europe
- Products
- Nippon BlueStar Plus - Model MWP04 - ...
Nippon BlueStar Plus - Model MWP04 -Prestained Protein Marker
The BlueStar PLUS Prestained Protein Marker is a three color protein standard with 12 prestained proteins covering a wide range molecular weights from 10 – 240 kDa. The Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins.
Three colour protein ladder (10 - 240 kDA)
- Broad range: 10 – 240 kDa
- 12 sharp bands with 3 colours (blue, red and green)
- 2 reference bands: 25 kDa (green) and 75 kDa (red)
- Ready to use: Supplied in a loading buffer for direct loading
- 500 µL: for 100 mini or 50 large gels
Figure 1: Molecular weight of the marker bands when using different buffer systems.
The molecular weight of the marker bands can vary depending on the gel/buffer system used. This is due to factors of the gel/buffer system that affect the running behaviour of proteins, e.g. migration rate, gel composition, pH-value (see FAQ for more information).
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, 2 % SDS, 0,2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
500 µL Protein Marker sufficient for 100 mini gels or 50 large gels.
Storage- Store at -20°C for 24 months.
- Stable at +4°C for 3 months.
- Stable at +25°C for 2 weeks.
- Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.
- Mix thoroughly, to ensure that the solution is homogeneous.
- Load the following volumes of the ladder on SDS-polyacrylamide gel
- 5 μl per well for mini-gels, 3 μl per well for blots
- 10 μl per well for large gels, 6 μl per well for blots
- Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis
- Monitoring of protein transfer onto membranes during Western blotting
- Sizing of proteins on SDS-polyacrylamide gels and Western blots