A. P. Buck Inc.

Buck Bio-CultureModel B30120 -Programmed Sampling for Bio-Contaminants Sampling Pump Kit

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The BUCK Bio-Culture pump, provides a selectable, continuous, constant sample flowrate and is simple to operate. No tools are required. Its quiet operation allows unobtrusive sampling in IAQ, medical, clean room, public and residential building applications.

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The front key pad is organized into three functional key groups: Power (ON-OFF), Timing (1, 2, 5, 10 minute samples), and Calibration (Cal mode, Increase flow, Decrease flow). Positive tactile feedback is provided by the keys. The BUCK Bio-Culture™ Pump has internal NiCad batteries to provide a full six hours continuous run time at 100LPM. The standard charger provides a battery recharge in 12-16 hours and allows the unit to operate on AC power; the optional FastOne Charger provides a battery recharge in one hour. End of sample notice is provided by the lit LED “Complete” and three short beeps.

  • Holds 90 mm agar Petri dish
  • Flow up to 120 LPM
  • 380 holes - 1 mm diameter
  • Timing routine of 1,2,5,10 minutes
  • Continuous operation for 6-8 hours*
  • Tripod mount to allow 90 degree sampling
  • Sampling head is easily sterilized with gas torch or autoclave
  • 4 X the flow rate of an Anderson sampler

The BUCK Bio-Culture Pump operates with an impeller type blower with very little backpressure resistance. This provides high flows and quiet operation. Traditional calibration devices have restricted openings which slow the pump speed. This specially designed Calibration Head fits on the BUCK Bio-Culture Pump replacing the sampling head to allow the flow selection by the user between 30 and 120 LPM. This flow should be calibrated with a blank agar plate installed. This Calibration is provided with a Certificate of Calibration which was performed with a NIST traceable Venturi Calibrator with a 1% accuracy.

Calibration:

  1. 1. Replace sampling head with the BUCK Bio-Culture Calibrator with attached head and pressure gauge as shown.
  2. 2. Push “ON”, then press “CAL” for 2 seconds to put pump into calibration mode. Adjust the flowrate using the ▲ and ▼ keys to the desired mm of water pressure to achieve the correct flow per minute. Correlate with the calibration chart on the side of the gauge. Fine adjustments are possible. Allow pump to stablize.
  3. 3. When flowrate is achieved, push “CAL” to accept setting, then turn pump off.
  4. 4. Verify the flow at anytime with the Calibration Head by selecting a 1, 2, 5 or 10 minute timming route. Press the OFF key to stop. If the verify flow was accurate entering “Cal” is not necessary.

Caution: Entering the “Cal” mode removes the previous setting to allow for a new flow rate.

BUCK Bio-Culture Sampling Steps
Before sampling, ensure the battery is fully charged and that the “Low Battery” LED is not on. For greatest accuracy, the pump should be calibrated once a day.

  1. Place pump in desired location.
  2. Immediately before sampling, open a 90mm Agar plate.
  3. Unscrew the sample head and position the plate within the mounts. Screw the head back on securely.
  4. Push the “ON” button to power up the BUCK Bio-Culture Pump and either: (a) continue to hold the “ON” button for four seconds to put pump in “Continuous” mode, or (b) push one of the programmed time keys (1, 2, 5, or 10 minutes) for an automatic timed sample.
  5. At the end of programmed sample period, pump will automatically shut off and “Complete” LED will be lit; under “Continuous” mode, push and hold “OFF” button for 2 seconds to shut off pump.
  6. Unscrew the head and remove the Petri dish.
  7. Replace the cap on the dish and send to an appropriate laboratory for analysis.

  • Flowrate: 30-120LPM constant flow
  • Accuracy: ± 5% of set point
  • Run Time:
    • 100 LPM: 8 hrs,
    • 120 LPM: 6 hrs
  • Size: (4.5 in H x 6 in W x 5.25 in D)
  • Weight: 42 oz. 2.6 lbs
  • Compatibility: 90mm Agar plate