µPulse® - Automated Tangential Flow Filtration (TFF) System for Sample Concentration & Diafiltration

Benefits:

• Walk-Away System
  Set your desired parameters and let the system do 95% of the process automatically until it is time to collect your sample; Remotely monitor via a web-based application.
• Fast
  Permeate flow rate up to 4x faster than centrifuges with dead-end filters.
• Scalable Process
  Whether you are trying to scale up or down, µPulse allows you to control all the parameters of an industrial TFF, at the research scale.
• Wide Range of Operating Volumes
  Concentrate from 100mL to 1mL with up to 250 mL of buffer exchange.
• Lowest Hold-up Volume in the Industry
  Our patent-pending pump design offers just 0.65 mL of hold-up volume. Up to 100% hold-up recovery with automated recovery options.
• Easy Maintenance
  All components in the fluid path are disposable consumables.
• Cost-Effective
  The µPulse consumable chips can be cleaned in place for re-use up to 300 mL of permeate (sample dependent).

Versatile for a Broad Range of Scientific Workflows:

• Nanoparticle Processing
  Efficiently process lipid nanoparticles, liposomes, and polymeric nanoparticles for drug delivery and therapeutic efficacy.
• Protein Processing
  Optimize protein workflows with fast, gentle concentration, formulation, desalting, and refolding.
• Bioconjugate Cleanup
  Ensure gentle and efficient removal of small unconjugated molecules from a variety of crude biomolecular labeling reactions.
• Nucleic Acid Processing
  Simplify in vitro RNA synthesis and concentrate plasmid or linear DNA with efficient buffer exchange using an easy-to-operate system.
• Harvesting
  Streamline harvesting of cells, extracellular vesicles, enzymes, and VLPs while maintaining high product yields and quality.
• Virus Processing
  Gently concentrate and exchange buffers for AAVs, bacteriophages, and lentiviruses, preserving structural integrity.
• Vaccine Development
  Optimize DNA, RNA, and polysaccharide vaccine formulation for stable, efficient, and cost-effective results.

Electrical

• Input: 110–240 V, 50–60 Hz
• Power: 50 W typical, up to 150 W max
• Supports all standard outlet types
• Connectivity: WiFi / Ethernet

Membrane

• Materials: Modified Polyethersulfone (mPES) or Regenerated Cellulose (RC)
• Membrane Area: 11 cm²
• Storage Solution: 20% Glycerol, 0.05% Sodium Azide
• Lifetime: Up to 300 mL total permeate volume

System

• Recommended Starting Volume: 5 – 100 mL
• Minimum Starting Volume: 1 mL
• Final Sample Volume: 0.5 mL with 0.65 mL recoverable hold-up
• Maximum Buffer Volume: 250 mL (in 50 mL increments)
• Volume Accuracy: ± 0.2 mL
• Suggested Max Sample Viscosity: 100 cP

Physical Dimensions

• Width: 263 mm (10.4”)
• Height: 233 mm (9.2”) / 342 mm (13.5”) with screen monitor
• Depth: 345 mm (13.5”)
• Weight: 10.8 kg (24 lbs)