Pyrotell-T - Reagent Turbidimetric Endotoxin Testing Kit

It can be used for a wide variety of tests, ranging from water testing to samples requiring high sensitivity, such as intrathecal products and those requiring high dilutions to overcome interference.

Sensitivity

When used in a Pyros Kinetix® Flex tube reader the maximum sensitivity is 0.001 EU/mL. The unique formulation of Pyrotell®-T allows a wide selection of standard curves to be used, giving the user flexibility, speed and ease in performing assays.

Sample to BET Ratio

The ratio of sample to BET is determined by personal preference and sample chemistry (interference patterns). Reconstituted Pyrotell®-T reagent is used at a sample to lysate ratio of 1:1 or 4:1 and volume of 100 μL/well (48 tests/vial) or 50 μL/well (96 tests/vial), respectively.

Performing the Test

The Pyrotell®-T sample mixture is incubated in an optical reader at 37±1°C and read at a desired wavelength depending on the instrumentation and user choice. The time of incubation is dependent on the lowest standard concentration in the standard curve. Software is used to analyze the standard curve and calculate the endotoxin concentrations.

Reconstitution

Pyrotell®-T may be reconstituted with 5 mL of LAL Reagent Water (LRW), Pyrosol® buffer, or Glucashield® buffer, depending on the demands of the sample being tested. Pyrosol® buffer provides improved kinetics and extra pH buffering capacity. Glucashield® buffer, a (1g3)-b-D-Glucan inhibiting buffer, is used to render the assay endotoxin specific.

Stability

Once reconstituted, Pyrotell®-T is stable for 24 hours, if stored at 2–8°C. Pyrotell®-T may be frozen once and will retain activity for as long as 3 months if stored at or below -20°C.

Packaging

Pyrotell®-T is available in multi-test vials. Each vial contains reagent for approximately 96 tests (when used with the Pyros Kinetix® Flex tube reader and 4:1 sample to BET ratio) or 48 tests (when used with 1:1 ratio and/ or in a microplate reader). It is recommended for use with the 0.5 μg/ vial Control Standard Endotoxin (CSE, E0005-1). Certificates of Analysis, specific to the Pyrotell®-T and CSE lot, can be obtained from ACC or online.