MedChemExpress - Model alpha-1,4-Galactosyltransferase (LgtC) -52725-57-2
alpha-1,4-Galactosyltransferase (LgtC) (A4GALT) is a glycosphingolipid-specific glycosyltransferase. alpha-1,4-Galactosyltransferase (LgtC) transfers a galactose to the alpha-1,4 position of lactosylceramide to form globotriaosylceramide. alpha-1,4-Galactosyltransferase (LgtC) can be used for the synthesis of P1 blood group antigens[1][2].MCE products for research use only. We do not sell to patients.
alpha-1,4-Galactosyltransferase (LgtC)
MCE China：alpha-1,4-Galactosyltransferase (LgtC)
Brand：MedChemExpress (MCE)
Cat. No.HY-E70047
CAS：52725-57-2
Synonyms：A4GALT
Storage：Please store the product under the recommended conditions in the Certificate of Analysis.
Shipping：Room temperature in continental US; may vary elsewhere.
Description：alpha-1,4-Galactosyltransferase (LgtC) (A4GALT) is a glycosphingolipid-specific glycosyltransferase. alpha-1,4-Galactosyltransferase (LgtC) transfers a galactose to the alpha-1,4 position of lactosylceramide to form globotriaosylceramide. alpha-1,4-Galactosyltransferase (LgtC) can be used for the synthesis of P1 blood group antigens.
In Vitro：Product InformationThis product is recombinantly expressed in E. coli, with an N-terminal His-tag, a molecular weight of 32 kDa, and a buffer composition of 20 mM Tris-HCl, pH 7.5, 5% glycerol. It can be used to transfer galactose to sugar chains with Gal as the non-reducing end. Instructions1. Add substrate according to the reaction system, 5 mM MgCl2, 1 mM DTT, and appropriately add buffer salt (such as Tris-HCl), adjust the pH to 7.5, and add appropriate amount of LgtC (according to the reaction requirements); 2. After mixing, incubate the sample at 37 ℃ for 2-4h. Notes When the system is scaled up, the incubation time or enzyme amount needs to be adjusted according to the actual situation, and can be mixed by low-speed shaking.
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References：
[1]. Kaczmarek R, et al. Evaluation of an amino acid residue critical for the specificity and activity of human Gb3/CD77 synthase. Glycoconj J. 2016 Dec;33(6):963-973. [Content Brief]
[2]. Iwamura K, et al. The blood group P1 synthase gene is identical to the Gb3/CD77 synthase gene. A clue to the solution of the P1/P2/p puzzle. J Biol Chem. 2003 Nov 7;278(45):44429-38. [Content Brief]
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