RayBio - Human/Mouse MAPK Phosphorylation Antibody Array 1 Kit

Antigen Information
Gene Symbols:AKT1 | CREB1 | GSK3A | GSK3B | HSPB1 | MAP2K1 | MAP2K3 | MAP2K6 | MAPK1 | MAPK14| MAPK8 | MTOR | RPS6KA1 | RPS6KA3 | RPS6KA4 | RPS6KB1 | TP53

Assay Format
Pathway: Akt SignalingHER/ErbB SignalingIGF SignalingJAK/STAT SignalingMAPK SignalingNFkB SignalingPI3K-AKT SignalingWnt/beta-Catenin Signaling
Number of Targets Detected:17
Species Detected:HumanMouse
Compatible Sample Types:Cell Culture SupernatantsCell LysatesPlasmaSerumTissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane

Product Specifications
Size:2, 4, or 8 Sample Kit

Product Features

• Easy to use
• No specialized equipment needed
• Compatible with nearly any liquid sample
• Proven technology (many publications)
• Highly sensitive (pg/ml)
• Sandwich ELISA specificity
• Higher density than ELISA, Western blot or bead-based multiplex

Application Notes
Suggested Application: Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

• Human/Mouse MAPK Phosphorylation Antibody Array C1 Membranes
• Blocking Buffer
• Detection Antibody Cocktail
• 1,000X HRP-Anti-Rabbit-IgG Concentrate
• 20X Wash Buffer I Concentrate
• 20X Wash Buffer II Concentrate
• 2X Cell Lysis Buffer Concentrate
• Detection Buffer C
• Detection Buffer D
• 8-Well Incubation Tray w/ Lid
• Protease Inhibitor Cocktail
• 100x Phosphatase Inhibitor Cocktail I
• Phosphatase Inhibitor Cocktail II
• Plastic Sheets
• Array Map Template
• Manual

Other Materials Required

• Pipettors, pipet tips and other common lab consumables
• Orbital shaker or oscillating rocker
• Tissue Paper, blotting paper or chromatography paper
• Adhesive tape or Saran Wrap
• Distilled or de-ionized water
• A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE`s ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

1. Block membranes
2. Incubate with Sample
3. Incubate with Detection Antibody Cocktail
4. Incubate with HRP-Conjugated anti-IgG
5. Incubate with Detection Buffers
6. Image with chemiluminescent imaging system
7. Perform densitometry and analysis

Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.