RayBio - Model C1 -Human Receptor Tyrosine Kinase Phosphorylation Antibody Array 1 Kit

Antigen Information
Gene Symbols:ABL1 | AXL | BLK | BMX | BTK | CSF1R | CSK | DDR2 | EGFR | EPHA1| EPHA2 | EPHA3 | EPHA4 | EPHA5 | EPHA6 | EPHA7 | EPHA8 | EPHB1 | EPHB2 | EPHB3 | EPHB4 | EPHB6 | ERBB2 | ERBB3 | ERBB4 | FER | FGFR1 | FGFR2 | FGR | FLT4 | FRK | FYN | HCK | IGF1R | INSR | ITK | JAK1 | JAK2 | JAK3 | KDR | KIT | LCK | LTK | LYN | MATK | MET | MUSK | NGFR | NTRK2 | PDGFRA | PDGFRB | PTK2 | PTK2B | RET | ROR1 | ROR2 | ROS1 | RYK | SLPI | SRMS | SYK | TEC | TEK | TIE1 | TNK1 | TNK2 | TXK | TYK2 | TYRO3 | ZAP70

Assay Format
Pathway: PI3K-AKT Signaling
Number of Targets Detected:71
Species Detected:
Human
Compatible Sample Types:
Cell Culture SupernatantsCell LysatesPlasmaSerumTissue Lysates
Design Principle:
Sandwich-based
Method of Detection:Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:Membrane

Product Specifications
Size:2, 4, or 8 Sample Kit

Product Features

• Easy to use
• No specialized equipment needed
• Compatible with nearly any liquid sample
• Proven technology (many publications)
• Highly sensitive (pg/ml)
• Sandwich ELISA specificity
• Higher density than ELISA, Western blot or bead-based multiplex

Species Detected
Human

Application Notes
Suggested Application: Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components

• Human RTK Phosphorylation Antibody Array C1 Membranes
• Blocking Buffer
• Wash Buffer 1
• Wash Buffer 2
• Biotinylated Anti-Phospho Tyrosine Detection Antibody
• Streptavidin-Conjugated HRP
• Detection Buffer C
• Detection Buffer D
• Lysis Buffer (1 x 5 ml)
• Protease Inhibitor Cocktail
• Phosphatase Inhibitor Cocktail II
• 8-Well Incubation Tray
• Plastic Sheets
• Array Templates
• Manual

Other Materials Required

• Pipettors, pipet tips and other common lab consumables
• Orbital shaker or oscillating rocker
• Tissue Paper, blotting paper or chromatography paper
• Adhesive tape or Saran Wrap
• Distilled or de-ionized water
• A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE`s ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

1. Block membranes
2. Incubate with Sample
3. Incubate with Biotinylated Detection Antibody Cocktail
4. Incubate with HRP-Conjugated Streptavidin
5. Incubate with Detection Buffers
6. Image with chemiluminescent imaging system
7. Perform densitometry and analysis

Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.