ReadiLink - Rapid Antibody Labeling Kits
AAT Bioquest ReadiLink™ Rapid Antibody Labeling Kits provide a quick and convenient method to label microscale volumes of antibodies with our superior iFluor®dyes, mFluor™ dyes, or other labels. The unique chemistry of ReadiLink™ kits enables researchers to effortlessly label and recover 100% of their antibodies without a purification step. Since ReadiLink™ conjugates are covalently labeled, they are stable for long-term storage. They are ideal for demanding applications, including fluorescent microscopy, multi-color imaging, multiplex flow cytometry, primary detection, IHC, FISH, ELISAs, and more.
- Label monoclonal antibodies, polyclonal antibodies, or other proteins
- Quick and simple two-step labeling protocol, no chemistry or conjugation experience necessary
- Useful for labeling 100 µg of antibody (two separate labeling reactions of 50 µg antibody)
- 100% antibody recovery, NO purification of the product is required
- Conjugates ready to use in 1 hr, with as little as ~10 minutes of hands-on time
- Choice of iFluor®dyes, mFluor™ dyes, Alexa Fluor®equivalent AF dyes, FITC, Cy3, Cy5, Cy7, BSA, KLH or biotin
- Avoid using any amine-containing buffers (e.g., Tris or glycine)
- Stabilizing proteins (e.g., BSA or gelatin) must be removed before labeling; for antibody labeling kits compatible with BSA, use ReadiLink™ xtra Rapid Antibody Labeling Kits
ReadiLink™ Rapid Antibody Labeling Kits utilize amine-reactive labels to prepare covalently labeled conjugates. These reactive labels selectively target and bind to primary amines, such as lysine residues, on the antibody of interest. To label your antibody, mix your antibody solution (concentration ~1 mg/mL) with the ReadiLink™ reaction buffer, add the mixture to the vial of pre-formulated label, and incubate for 30 to 60 minutes. Lastly, add the TQ™-Dyed Quench Buffer to your mixture and mix well. This part is key as it eliminates both background interference and the column purification step. After 10 minutes of incubation, your conjugates are ready to use.