CapeCod Pyrochrome - Reagent Chromogenic Endotoxin Testing Kit

Sensitivity

The maximum sensitivity of Pyrochrome® is 0.001 EU/mL when run in Pyros Kinetix® Flex tube reader or incubating microplate reader with Glucashield® Buffer.

Sample to BET Ratio

In the Pyros Kinetix® Flex tube reader, Pyrochrome® can be used at an economical ratio of 4:1 using 50 μL of reagent per well or at 1:1 using 100 μL/well. In a microplate reader, the reagent is used at a ratio of 1:1 and a volume of 50 μL/well (60 tests/vial) or 100 μL/well (30 tests/vial).

Performing the Test

The Pyrochrome® sample mixture is incubated in an optical reader at 37±1°C and read at a wavelength of 405 nm. No pre-incubation is required and results can be available within 1 hour. However, time to results is dependent on the required assay sensitivity. Software will analyze the data to provide endotoxin results.

Reconstitution

Pyrochrome® lysate is reconstituted with an optimized Pyrochrome® reconstitution buffer (C1500-5). Pyrochrome® can also be reconstituted with Glucashield® buffer (CG1500-5), a (1g3)-b-D-Glucan inhibiting buffer, to render the assay endotoxin specific.

Stability

Once reconstituted, Pyrochrome® is stable for 8 hrs. when stored at 2–8ºC.

Packaging

Pyrochrome® is offered with a choice of reconstitution buffer and is recommended for use with the 10 ng/vial Control Standard Endotoxin (CSE, EC010-5). Certificates of Analysis, specific to the Pyrochrome® and CSE lot, can be obtained from ACC or online at www.acciusa.com.

Pyrochrome® Chromogenic Test Kit
3.2 mL/vial (approx. 60 tests/vial)
Each kit contains Pyrochrome® and a buffer

• #C1500-5 Pyrochrome® with Reconstitution Buffer 5 pack (300 tests)
• #C1500-25 Pyrochrome® with Reconstitution Buffer 25 pack (1500 tests)
• #CG1500-5 Pyrochrome® with Glucashield® Buffer 5 pack (300 tests)
• #CG1500-25 Pyrochrome® with Glucashield® Buffer 25 pack (1500 tests)