Reagents to power eProtein Discovery
Specially formulated reagents power your workflow from DNA preparation to protein expression screening, purifiability assessment and then off cartridge scale up. Features and Benefits: The reagents within the eProtein DiscoveryTM system allow you to optimize protein obtainability by characterizing and purifying different combinations of DNA constructs and expression conditions. Our system will screen 192 different combinations in 24 hours for you to select the optimal conditions to scale up and get protein. Our eProtein DiscoveryTM software will guide you in creating the panel of DNA constructs and reagents to power your experiment. Our complete reagent package includes design and ordering of DNA, simplifying your workflow.
Customize DNA constructs (eGene) design with pre-set fusion tags to optimize expression.
Optimize expression with different reagent formulations based on your protein needs.
Scale up expression and purification of your proteins of choice to use in downstream applications.
eGene™ Prep Kit: Solubility tags: Multiple solubility tag options added to N-terminus
eGene™ Prep Kit: Purification tag: Strep added to C- terminus
eGene™ Prep kit: Protease cleavage sites: TEV and 3C protease sites added. Option for solubility screening after tag removal
Cell-free Core Reagents: E. coli derived Cell Free Protein Synthesis (CFPS) reagents
Additives: Redox regulators: Promote disulfide bond formation in your proteins
Additives: Chaperones: Aid in protein folding and stability
Additives: Metal ions: Promotes correct folding of metalloproteins
Additives: Co-factors: Stabilizes proteins
Purification reagents: Strep purification beads and purification reagents included
Scale Up Kits: Prototype-scale kits provided for scale up expression in tubes. Additional scale up material available to purchase
