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Hamilton - Model PRP-C18 -Reversed-Phase HPLC Columns
FromHamilton Company
PRP-C18 columns have genuine pH and chemical stability. The stationary phase stands up to prolonged exposure to concentrations as high as 1 M NaOH and 1 M H2SO4, with no measurable decrease in performance. The support does not strip, bleed, or dissolve at any pH. It can therefore perform reliably and reproducibly throughout the extended life of the column, regardless of mobile phase conditions. Unlike a silica C18 column, the PRP-C18 is resistant to temperatures above 100°C.
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Application Examples
Organic compounds including:
- small molecules (< 2,000 mw)
- pharmaceuticals
- steroids
- halides
- vitamins
- amino acids
- herbicides
- high pH applications
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Separation of Selected Components of Ylang-Ylang Oil (PRP-C18)In this study of an essential oil, we have developed an HPLC method for the separation of 6 well-known markers for the determination of purity of Ylang-Ylang oil.
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Strongly Basic Drug Compounds (PRP-C18)In this study, 9 structurally-diverse, strongly basic drug compounds are resolved in less than 2 minutes using a high pH mobile phase and a fast acetonitrile gradient at 80°C.
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Separation of Basic Drug Compounds (PRP-C18)Rapid separations under alkalin pH conditions.
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Separation of Five Steroids (PRP-C18)A reversed-phase HPLC method was developed for separation of five steroid hormones with partition coefficients ranging from 1.47 (cortisone) to 4.5 (pregnenolone) on a Hamilton PRP-C18 HPLC column.