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- RiboGrip - RNase Inhibitor
RiboGrip - RNase Inhibitor
FromSolis BioDyne
RiboGrip™ RNase Inhibitor is an in silico-designed protein-based ribonuclease inhibitor, which inactivates RNase A. RiboGrip™ inhibits the activity of ribonuclease A by noncovalently binding in a non-competitive mode at a ratio of 1:1. This novel inhibitor has enhanced stability at room temperature with no loss of activity for up to 1 month.
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- included in our cDNA synthesis kits and 1-step RT-qPCR kits
- reaction set-up and shipment without ice
Preventing RNA degradation in the following applications:
- First-strand cDNA synthesis
- RT-PCR
- RT-qPCR
- in vitro transcription and translation
- Concentration: 40 U/µl
- Source: Purified from an E.coli strain that carries an overproducing plasmid containing a RiboGrip™ RNase Inhibitor gene.
- Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5, 100 mM KCl, 0.1 mM EDTA, 5 mM DTT and stabilizers.
- Recommendations for use: RiboGrip RNase Inhibitor™ is used generally at a final reaction concentration of 1 U/μl. Inactivated by heating at 85°C for 5 min.
- Included in FIREScript® and SOLIScript® cDNA Synthesis Kits and Mixes, and in all SOLIScript® 1-step RT-PCR kits
