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- Alator - Model I104 - rRibonuclease ...
Alator - Model I104 -rRibonuclease Inhibitor VI (cGMP)
Source: E.coli. Size: Bulk. Concentration: 40 U/µL. Storage Buffer: 20 mM HEPES-KOH (pH 7.6) at 40C., 50 mM KCl, 8 mM DTT and 50% glycerol (v/v). Purity: >95% by SDS-PAGE.
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- Unit Definition: One unit is the amount of enzyme required to inhibit by 50% the activity of 5 ng of RNase A at 25°C (This
- inhibitor activity is determined by its ability to inhibit hydrolysis of cyclic cytidine 2`: 3`-monophosphoric acid). 200 units of enzyme give 50% inhibition of 1 ug of RNase A.
- Quality Control:
- Nuclease activity: 1 ug EcoR I /Hind III fragments of lambda DNA were incubated with enzyme in 50 ul of 50 mM Tris-HCl, 10 mM MgCh, 0.1 mM EDTA, and 7 mM p-Mercaptoethanol, pH 7.5 for 1 hour at 37°C. > 400 units of enzyme showed no alteration of the banding pattern.
- Nicking Activity: 1 ug supercoiled DNA were incubated with enzyme in 50 ul of 50 mM Tris-HCl, 10 mM MgCh, 0.1 mM EDTA, and 7 mM p-Mercaptoethanol, pH 7.5 for 1 hour at 37°C. > 400 units of enzyme showed no alteration of the no relaxation of supercoiled DNA.
- RNase Activity: 5 ug of MS2 RNA were incubated with enzyme in a final volume of 50 ul for 1 hour at 37°C. > 400 units of enzyme showed no degradation of MS2 RNA.
- RNase Activity after inactivation: 5 ug of MS2 RNA were incubated with enzyme for 10 minutes at 65°C following an incubation for 1 hour at 37°C in a final volume of 50 ul. Up to 160 units of enzyme showed no degradation of MS2 RNA.
- Expiration: Twelve months from shipment date
- Storage and Handling: -20°C