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Sage Science - Model BluePippin - DNA ...

Sage Science - Model BluePippin -DNA Size Selection System

The BluePippin DNA Size Selection System by Sage Science is designed to provide precise selection and collection of DNA fragments ranging from 100 base pairs to 50 kilobases. Utilizing pulsed-field electrophoresis, it facilitates the separation of high molecular weight DNA, crucial for long-range genomic studies. Users can set specific size thresholds via software, allowing high-pass filtering to collect fragments above the desired size. This system enhances process efficiency by accommodating up to five samples per gel cassette, minimizing cross-contamination risks. Available in different agarose concentrations, the cassettes offer flexible size selection. The BluePippin employs constant voltage settings with options for pulsed fields to cater to a variety of applications. Integrated with a built-in CPU and optical detection capabilities, it simplifies operations by eliminating the need for external computing devices. The system supports faster run times, high input capacity, and ensures reliable DNA size selection for advanced research applications.

Size-Select DNA Fragments up to 50kb

Collect HMW DNA with High-Pass Filtering

The BluePippin has the DNA capabilities of the Pippin Prep, plus the extra benefit of pulsed-field electrophoresis for resolving and collecting high molecular weight DNA. For long-range genomic applications, high-pass filtering allows users to collect all fragments above a size threshold set by the user.

Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer. Up to 5 samples/gel cassette may be run, with no possibility of cross contamination.

Benefits of the BluePippin System

Saves time and effort compared to manual gel purification
Sample-to-sample reproducibility streamlines research
Higher sample quality improves downstream analysis

Key Downstream Technologies

Mate-pair sequencing
Single molecule sequencing
Bar-coded linked reads

Pre-Cast Agarose Gel Cassettes for DNA Size Selection

Gel cassettes for DNA selections are available in four agarose concentrations to provide a full range of size selection options between 100bp up to 50kb. For smaller fragment sizes, internal standards may be used for higher accuracy and increased sample capacity (from 4 to 5 samples/cassette). Higher molecular weight fragments require a dedicated lane to run a calibration marker. Run times are as little as 30 minutes, To resolve very large DNA fragments, pulsed-field runs may last several hours. View the BluePippin cassette reference chart to explore the DNA size selection options and run times.

Standard DNA Size Selection Cassettes

3% agarose, 100-250bp
2% agarose, 100-600bp
1.5% agarose, 250bp – 1.5kb
0.75% agarose, 1-50kb
0.75% agarose, >4-40kb High-Pass
0.75% agarose, “Range+T” 9-30kb Size Selection

High Pass Plus Cassettes

Faster run times and higher (10ug) input capacity

>15KB and >20kb High Pass

Downstream Technologies

miRNA isolation
DNA sequencing, RNA-seq, genotyping
DNA sequencing, ChIP-seq
Long-read sequencing (PacBio, Oxford Nanopore)

Instrument
The BluePippin instrument includes a monitor, keyboard, and mouse. A CPU is built in; an external PC is not required.

Blue Pippin Specs :

Electrophoresis voltage : 25V, 100V or 150V constant, 100V pulsed field
Optical detection : 470 nm excitation, 525 nm emission
Power requirements : 100-240 VAC, 2.5 A, 50-60 Hz
Weight : 15 lbs / 7 kg
Dimensions : 7h x 11w x 21d (in) | 18h x 28w x 53 d (cm)