Sep-Pak SPE - Sample Extraction Instruments

Sep-Pak® 96-Well Plates are available with sorbents that have been proven for years in Sep-Pak cartridges. Introduced for high volume applications, the 96-well plate design has been optimized for robotic and automated sample handling systems as well as for standard vacuum manifolds. Sep-Pak plates are available with different sorbent mass selections to optimize sample loading.

Sep-Pak 96-well plates are extensively tested for quality. A certificate of analysis describes the performance of the pre-bonded and bonded silica phases in terms of ligand density, cleanliness and chromatographic selectivity. The plates are manufactured and tested to ensure consistent flow properties across the 96 wells.

Revered or Normal Phase

Amino Propyl (NH2)

• Silica-based polar bonded phase with basic character
• Can be used as a polar sorbent, like silica, with different selectivity for acidic/basic analytes or as weak anion exchanger in aqueous medium
• Applications include phenols and phenolic pigments, petroleum fractionation, saccharides and drugs and metabolites

Cyano Propyl (CN)

• Silica-based polar bonded phase
• Can be used as less polar alternative to silica in normal-phase applications or as less hydrophobic alternative to C18 or C8 in reversed-phase
• Applications; typical applications include drugs, drug metabolites and pesticides

Diol –

• Silica-based polar bonded phase with neutral character
• Can be used as an alternative to silica in normal phase applications, where the acidic character of silica is undesirable or as very weakly interacting phase in aqueous applications
• Applications include antibiotics from cosmetics; isolation of proteins or peptides by hydrophobic interaction chromatography

Normal Phase

Silica

• Polar sorbent, used primarily to adsorb analytes from non polar solvents like hydrocarbons, chloro- or fluoro-substituted hydrocarbons or less polar esters and ethers
• Elution with more polar solvents like polar esters, ethers, alcohols, acetonitrile or water
• The binding mechanism can be hydrogen bonding or dipole-dipole interaction
• Silica can also be used in aqueous medium as a cation exchanger of intermediate strength.

Alumina (A, B & N)

• Similar in use to silica; available in acidic, basic and neutral high activity grades
• Alumina also exhibits specific interactions with the pi-electrons of aromatic hydrocarbons, making it useful for applications like crude oil fractionation
• Acidic and basic grades can also be used as low-capacity ion-exchangers

Florisil®

• Polar, highly active, weakly basic sorbent for adsorption of low to moderate polarity species from nonaqueous solutions
• Specifically designed for the adsorption of pesticides using official AOAC and EPA methods
• Other applications include polychlorinated biphenyls (PCB’s) in transformer oil

C18

• Silica-based bonded phase with strong hydrophobicity
• Used to adsorb analytes of even weak hydrophobicity from aqueous solutions
• Typical applications include drugs and their metabolites in serum, plasma or urine, desalting of peptides, trace organics in environmental water samples, organic acids in beverages
• Similar in behavior to reversed-phase HPLC columns

tC18

• Silica-based bonded phase with strong hydrophobicity; trifunctional bonding chemistry gives it an increased hydrolytic stability over C18
• Applications similar to C18

C8

• Silica-based bonded phase with moderate hydrophobicity
• Used for methods requiring less retention than C18;
• Typical applications include drugs and their metabolites in serum,plasma or urine, peptides in serum, plasma

tC2 –

• Silica-based bonded phase with low hydrophobic character
• Used for methods requiring less retention than C8
• Applications are similar to C18 and C8

Porapak™ RDX

• Specially cleaned polymeric poly(divinylbenzene-vinylpyrrolidone) resin with hydrophobic character
• Can be used as an alternative to octadecyl-bonded silica for preparation of analytes that weakly adsorb to silica-based reversed-phase sorbents
• Compatible with sample or eluents at high and low pH
• Specifically designed for the concentration of high explosives in aqueous samples

Accell™ Plus QMA –

• Silica-based hydrophilic strong anion-exchanger with large pore-size; extraction of anionic analytes in aqueous and non-aqueous solutions
• Due to the large pore-size, it is excellent for the isolation of anionic proteins, e.g., immunoglobulins, enzymes
• Other applications include the removal of acidic pigments from wines, fruit juices and food extracts, isolation of phenolic compounds, peptide pool fractionations

Accell™ Plus CM –

• Silica-based hydrophilic weak cation-exchanger with large pore-size
• Extraction of cationic analytes in aqueous and non-aqueous solutions
• Due to the large pore-size, it is excellent for the isolation of cationic proteins
• Other applications include pesticides, herbicides, steroids

PSA (primary-secondary amine) SPE sorbent is a uniquely versatile sorbent exhibiting selectivity for normal-phase, weak anion-exchange, HILIC applications. When comparing to PSA to aminopropyl sorbents, the selectivity of PSA is similar; however, the two amine groups in the primary and secondary positions give PSA increased ion-exchange capacity and improved retention. When used as a normal-phase sorbent the higher carbon content of the PSA compared to aminopropyl offers increased non-polar interactions.

Available in all Sep-Pak® formats including carbon black/PSA tandems cartridges.

Primary-Secondary Amine (PSA)

• A weak anion exchanger with pKa 10.1 and 10.9
• Stronger anion exchanger than aminopropyl
• Strong affinity and high capacity for fatty acids, organic acids, sugars
• Bidentate ligand allows for chelation
• Mean ion-exchange capacity 1.75 meq/g