Shimadzu - Model UV-1900 - Double-Beam UV-VIS Spectrophotometer
The UV-1900 double-beam UV-Vis spectrophotometer provides the optimum balance of performance and usability, with high resolution, low stray light, an ultra-fast scan function, and an easy-to-use interface.
The UV-1900 on-screen user interface includes large, easy-to-see icons deployed on a black background, providing a clear picture of the instrument settings on the display. In addition, the large, easy-to-see icons improve intuitive understanding, which enables users to quickly become familiar with the operations. The user interface is designed to minimize transitions between windows, so users do not get confused during the operations.
In quantitation mode on the UV-1900, the stages of the entire measurement process and the current status are always shown on the display. As a result, users know immediately what to do in the next step.
The control panel design is based on ergonomics and positioned at the very best viewing angle for the user. Users can operate easily with any posture. In addition, a stylus pen is equipped next to the panel, so users can switch easily between operations using their fingers or the stylus pen.
Instrument Validation Functions Compliant with JP, USP, and EP
This instrument can not only run checks for nine JIS items, but also those stipulated in the Japanese Pharmacopoeia (JP), United States Pharmacopeia (USP), and the European Pharmacopoeia (EP). Naturally, the hardware is also compliant with the specifications required by each Pharmacopeia. In addition, the check conditions can be saved. As a result, once the conditions are saved, checks can be performed easily just by calling them up as needed. Check results can also be saved.
Resolution of 1 nm, the Highest in its Class
In addition to achieving a resolution of 1 nm, the highest in its class, by using a monochromator with a Czerny-Turner mounting, the UV-1900 also features a compact, bright optical system. The instrument is more than capable of meeting the wavelength resolution required in the European Pharmacopoeia.
An external control security function has been added to provide more support for compliance with regulations. Three user authority levels, 'Administrator', 'Developer', and 'Operator', can be set for instrument users.
Support for FDA 21 CFR Part 11, PIC/S GMP Guidelines and Other Regulations and Guidelines
Ensuring the integrity of data (database management), including the user management, user authority management, and data audit trails required for compliance with FDA 21 CFR Part 11, PIC/S GMP guidelines, and other ER/ES regulations, is possible.
Spectra can be acquired as fast as 29,000 nm/min. Ultra-fast scan is effective in tracking chemical reactions in a short time. In addition to the absorbance change at specified wavelengths, spectra can also be acquired in a short time with the UV-1900. Therefore, more detailed behavior can be investigated by observing spectra with the UV-1900. The figures below show the analysis of particle agglomeration process when salts are added to silver nanoparticles. Measurements of the 300 to 700 nm region were performed in ultra-fast scan mode. In addition to the decrease of absorbance at 400 nm and the increase of absorbance at 480 nm, the temporal changes of spectra can also be observed.
Low Stray Light
Stray light is at 0.5 % max. (198 nm), twice as low as the performance level of the UV-1800. With this stray light reduction, accurate measurements are possible up to the vicinity of 2 Abs even in the ultraviolet region. In addition, high-concentration samples can be quantified accurately. The figure on the right is a calibration curve for acetic acid, created with absorbance at 200 nm. The correlation coefficient is 0.9997, and correct measured values are obtained even in the vicinity of 2 Abs. Linearity will be lost in the high absorbance region due to the stray light.
High Reproducibility and Repeatability Accuracy
The photometric repeatability accuracy is 0.0002 Abs max. (0.5 Abs and 1.0 Abs), an improvement of five times over the performance level of the UV 1800. With this high photometric repeatability accuracy, variance in the measurement results is suppressed, enabling more accurate quantitation and the detection of low-concentration samples.
The figure below is a calibration curve for caffeine, created with absorbance at 273 nm. The calibration curve has an Abs = 0.0528 Conc., the lower limit of quantitation determined from the standard deviation is 0.0051 mg/L at a point where it would be 0.051 mg/LNote for the UV-1900.
USB memory can be connected directly to the UV-1900.
Measures the photometric value at a single wavelength or multiple (up to eight) wavelengths.
Measures a sample spectrum using wavelength scanning.
Generates a calibration curve from the measurement of standards, and then calculates the concentrations of unknowns.
Measures absorbance changes as a function of time, and obtains the enzymatic activity values. The kinetics measurement method or the rate measurement method can be selected.
5. Time Course
Measures changes over time in photometric values at a specified wavelength.
Quantifies DNA or protein concentrations.