SOLIScript - 1-Step Probe Kit

Convenient kit format for performing highly specific cDNA synthesis and probe-based qPCR in a single tube. The kit contains qPCR mix, SOLIScript^® RT enzyme mix with RiboGrip™ RNase inhibitor, and nuclease-free water.

• cDNA synthesis up to 60°C for superior specificity
• suitable for singleplex and duplex assays
• reaction set-up and shipment without ice
• high specificity and sensitivity
• one kit for all cyclers
• RNase inhibitor included in kit

• Detection and quantification of RNA targets with qPCR
• DNA/LNA hydrolysis probe based assay
• Suitable for singleplex or duplex reactions

• Sample type: RNA
• Product format: Reagent kit 
• No of tubes: three tubes
• Detection type: Probe-based
• Reference dye: reference dye based on ROX
• Compatible real-time instruments: The  Kit is compatible with most qPCR cyclers that do or do not require an internal reference dye (e.g. ROX) for normalization of fluorescent signal. Check Your cycler!

• 5x One-step Multiplex Probe Mix (ROX) with HOT FIREPol^® DNA polymerase, reaction buffer, dNTPs including dUTP), 15 mM MgCl₂ (1x qPCR solution - 3 mM MgCl₂), internal reference based on ROX dye
• 40x One-step SOLIScript^® Mix with SOLIScript^® Reverse Transcriptase and RiboGrip™ RNase Inhibitor
• Water, nuclease free