Nikon - Model N-SIM S -Super-Resolution Microscope System

High-speed super-resolution imaging at 15 fps

Nikon’s new high-speed structured illumination system utilizes a novel pattern modulation technology to generate fast and precise switching of illumination patterns. The N-SIM S achieves incredible acquisition speeds (up to 15 fps*), enabling super-resolution time-lapse imaging of live cells and intracellular dynamics.

Live-cell imaging at double the resolution of conventional light microscopes

The N-SIM S utilizes Nikon`s innovative approach to Structured Illumination Microscopy technology. By pairing this powerful technology with Nikon’s renowned objectives, which achieve an unparalleled numerical aperture of 1.49, the N-SIM S nearly doubles (to approximately 115 nm*) the spatial resolution of conventional light microscopes, enabling detailed visualization of minute intracellular structures and their interactions.

Automatic switching between illumination modes

The newly-developed high-speed structured illumination technology enables fast acquisition rates, automatic switching between illumination modes, and automated optimization of structured illumination patterns for different wavelengths and magnifications. This expanded automation enables fast 2-color TIRF-SIM imaging, as well as multiplexing of different SIM modalities. The N-SIM S provides easy-to-use, streamlined workflows, whether it be for single-mode or multi-modal imaging experiments.

Acquire larger fields of view

N-SIM S can acquire super-resolution images with a large field of view of 66 µm square. This larger imaging area enables very high throughput for applications/samples that benefit from larger fields of view, such as a neurons, reducing the amount of time and effort required to obtain data.

Various observation modesTIRF-SIM/2D-SIM mode

This mode captures super-resolution 2D images at high speed with incredible contrast. The TIRF-SIM mode enables Total Internal Reflection Fluorescence observation at double the resolution of conventional TIRF microscopes, facilitating a greater understanding of molecular interactions at the cell surface.

3D-SIM mode

The 3D-SIM mode generates structured illumination patterns in three dimensions to deliver a two-fold improvement in lateral and axial resolutions. Two reconstruction methods (“slice” and “stack”) are available to optimize results according to application requirements (e.g. sample thickness, speed, etc.). Slice reconstruction is suitable for imaging living cells at specific depths, as it allows axial super-resolution imaging with optical sectioning at 300 nm resolution. Stack reconstruction, based on Gustafsson’s theory, is suitable for acquisition of volume data as it can image thicker specimens with higher contrast than slice reconstruction.