Sword Nuc-Cyte - DNA-Binding Assays
In response to our client’s needs, Sword Method Development Scientists have developed our proprietary Nuc-Cyte™ DNA-Binding Platform for the identification and quantitation of cellular transcription factors.
Transcription factors (TFs) are cellular proteins that regulate the transcription of DNA by binding to specific nucleic acid regulatory sequences within genes.
Their role in the regulation of gene expression is critical to fundamental cellular processes, such as cell differentiation, development, growth, replication, survival, and death.
To promote the accurate and precise detection and quantitation of TFs, we developed the Nuc-Cyte™ Platform for DNA Binding Assays (the Nuc-Cyte™ Platform). The Nuc-Cyte™ Platform consists of:
- A synthetic, biotinylated oligonucleotide (oligo) whose nucleotide sequence corresponds to the binding sequence for the specific TF of interest.
- A primary antibody (specific for the TF-oligo complex)
- A secondary antibody conjugated to horseradish peroxidase (HRP)
- Our proprietary Sword Swift™ Detection Reagents that generate a resonance Raman based assay signal with excellent to signal-to-noise ratio and stability (for up to 120 minutes at room temperature).
Nuc-Cyte™ DNA-binding immunoassays are:
- Versatile: the assay format can be readily adapted to quantitate different TFs by utilizing different oligos specific for different TFs
- Rapid: can be completed within 4 to 6 hours
- High throughput: multiple samples can be analyzed simultaneously in a microtiter plate-based format
- Highly sensitive, accurate, and precise: due to the use of our Sword Swift™ Detection Reagents, Nuc-Cyte™ DNA-binding immunoassays highly are sensitive, accurate, and precise in comparison to colorimetric-based immunoassays.
